Hilbrant, Maarten, Horn, Thorsten, Koelzer, Stefan and Panfilio, Kristen A. (2016) The beetle amnion and serosa functionally interact as apposed epithelia. eLife, 5 . e13834. doi:10.7554/eLife.13834 ISSN 2050-084X.

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Abstract

Unlike passive rupture of the human chorioamnion at birth, the insect extraembryonic (EE) tissues – the amnion and serosa – actively rupture and withdraw in late embryogenesis. Withdrawal is essential for development and has been a morphogenetic puzzle. Here, we use new fluorescent transgenic lines in the beetle Tribolium castaneum to show that the EE tissues dynamically form a basal-basal epithelial bilayer, contradicting the previous hypothesis of EE intercalation. We find that the EE tissues repeatedly detach and reattach throughout development and have distinct roles. Quantitative live imaging analyses show that the amnion initiates EE rupture in a specialized anterior-ventral cap. RNAi phenotypes demonstrate that the serosa contracts autonomously. Thus, apposition in a bilayer enables the amnion as 'initiator' to coordinate with the serosa as 'driver' to achieve withdrawal. This EE strategy may reflect evolutionary changes within the holometabolous insects and serves as a model to study interactions between developing epithelia.

Item Type:	Journal Article
Alternative Title:
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Journal or Publication Title:	eLife
Publisher:	eLife Sciences Publications Ltd.
ISSN:	2050-084X
Official Date:	29 January 2016
Dates:	Date Event 29 January 2016 Published 28 January 2016 Accepted
Volume:	5
Article Number:	e13834
DOI:	10.7554/eLife.13834
Status:	Peer Reviewed
Publication Status:	Published

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