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Late extraembryonic morphogenesis and its zenRNAi-induced failure in the milkweed bug Oncopeltus fasciatus
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Panfilio, Kristen A. (2009) Late extraembryonic morphogenesis and its zenRNAi-induced failure in the milkweed bug Oncopeltus fasciatus. Developmental Biology, 333 (2). pp. 297-311. doi:10.1016/j.ydbio.2009.06.036 ISSN 0012-1606.
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Official URL: http://dx.doi.org/10.1016/j.ydbio.2009.06.036
Abstract
Many insects undergo katatrepsis, essential reorganization by the extraembryonic membranes that repositions the embryo. Knockdown of the zen gene by RNA interference (RNAi) prevents katatrepsis in the milkweed bug Oncopeltus fasciatus. However, the precise morphogenetic defect has been uncertain, and katatrepsis itself has not been characterized in detail. The dynamics of wild type and zenRNAi eggs were analyzed from time-lapse movies, supplemented by analysis of fixed specimens. These investigations identify three zenRNAi defects. First, a reduced degree of tissue contraction implies a role for zen in baseline compression prior to katatrepsis. Subsequently, a characteristic ‘bouncing’ activity commences, leading to the initiation of katatrepsis in wild type eggs. The second zenRNAi defect is a delay in this activity, suggesting that a temporal window of opportunity is missed after zen knockdown. Ultimately, the extraembryonic membranes fail to rupture in zenRNAi eggs: the third defect. Nevertheless, the outer serosal membrane manages to contract, albeit in an aberrant fashion with additional phenotypic consequences for the embryo. These data identify a novel epithelial morphogenetic event – rupture of the ‘serosal window’ structure – as the ultimate site of defect. Overall, Oncopeltus zen seems to have a role in coordinating a number of pre-katatreptic events during mid embryogenesis.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Developmental Biology | ||||
| Publisher: | Academic Press | ||||
| ISSN: | 0012-1606 | ||||
| Official Date: | 15 September 2009 | ||||
| Dates: |
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| Volume: | 333 | ||||
| Number: | 2 | ||||
| Page Range: | pp. 297-311 | ||||
| DOI: | 10.1016/j.ydbio.2009.06.036 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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