The Library
The role of optical projection in the analysis of membrane fluctuations
Tools
Rautu, S Alex, Orsi, Davide, Di Michele, Lorenzo, Rowlands , George, Cicuta, Pietro and Turner, Matthew S. (2017) The role of optical projection in the analysis of membrane fluctuations. Soft Matter, 13 (19). pp. 3480-3483. doi:10.1039/c7sm00108h ISSN 1744-683X.
|
PDF
c7sm00108h.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution. Download (1776Kb) | Preview |
Official URL: https://doi.org/10.1039/c7sm00108h
Abstract
The spectral analysis of thermal fluctuations, or flickering, is a simple and non-invasive method widely used to determine the mechanical properties of artificial and biological lipid membranes. In its most common implementation, the position of the edge of a cell or vesicle is tracked from optical microscopy videos. However, a systematic disagreement with X-ray scattering and micromechanical manipulation data has brought into question the validity of the method. We present an improved analysis protocol that resolves these discrepancies by accounting for the finite vertical resolution of the optics used to image fluctuations.
Item Type: | Journal Article | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QC Physics | |||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Physics | |||||||||
SWORD Depositor: | Library Publications Router | |||||||||
Library of Congress Subject Headings (LCSH): | Soft condensed matter -- Research, Lipid membranes, Fluctuations (Physics), Microscopy | |||||||||
Journal or Publication Title: | Soft Matter | |||||||||
Publisher: | Royal Society of Chemistry | |||||||||
ISSN: | 1744-683X | |||||||||
Official Date: | May 2017 | |||||||||
Dates: |
|
|||||||||
Volume: | 13 | |||||||||
Number: | 19 | |||||||||
Page Range: | pp. 3480-3483 | |||||||||
DOI: | 10.1039/c7sm00108h | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Date of first compliant deposit: | 18 November 2021 | |||||||||
Date of first compliant Open Access: | 18 November 2021 | |||||||||
RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year