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Expression of nitric oxide synthase and, effect of substrate manipulation of the nitric oxide pathway in mouse ovarian follicles
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UNSPECIFIED (2004) Expression of nitric oxide synthase and, effect of substrate manipulation of the nitric oxide pathway in mouse ovarian follicles. HUMAN REPRODUCTION, 19 (1). pp. 30-40. doi:10.1093/humrep/deh032
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Official URL: http://dx.doi.org/10.1093/humrep/deh032
Abstract
BACKGROUND: Nitric oxide (NO) is a cell messenger with multiple actions in different biological systems, implicated in the control of follicle and oocyte function. NO is formed from L-arginine by isoforms of nitric oxide synthase (NOS) via N-G-hydroxy-L-arginine, with L-citrulline as a byproduct. This study aimed to show how modulation of NO by manipulating NOS substrates would affect mouse follicle growth and ovulation in vitro, where vascular effects of NO are attenuated. METHODS: Immunohistochemistry [endothelial (eNOS) and inducible (iNOS)] and in situ hybridization (iNOS) were applied on mouse ovaries. Cultured follicles were also stained for iNOS by immunohistochemistry. For follicles cultured in the presence or absence of L-arginine, the ability of L-citrulline or N-G-hydroxy-L-arginine to substitute for L-arginine was assessed in terms of follicle growth and ovulation. RESULTS: iNOS and eNOS were localized in oocytes and theca, with some staining in granulosa. iNOS mRNA occurred predominantly in granulosa and oocyte. Omission Of L-arginine significantly reduced follicle survival and ovulation. Partial compensation for L-arginine withdrawal was achieved with L-citrulline and N-G-hydroxy-L-arginine. Specific abnormalities of follicle growth were noted. CONCLUSIONS: NOS is present in mouse follicles, and its action is necessary at a local level for normal follicle development in vitro. Reduced growth, persistent basement membranes and reduced ovulation were associated with in vitro disruption of NO.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | R Medicine > RG Gynecology and obstetrics | ||||
| Journal or Publication Title: | HUMAN REPRODUCTION | ||||
| Publisher: | OXFORD UNIV PRESS | ||||
| ISSN: | 0268-1161 | ||||
| Official Date: | January 2004 | ||||
| Dates: |
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| Volume: | 19 | ||||
| Number: | 1 | ||||
| Number of Pages: | 11 | ||||
| Page Range: | pp. 30-40 | ||||
| DOI: | 10.1093/humrep/deh032 | ||||
| Publication Status: | Published |
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