UNSPECIFIED. (2004) Expression of nitric oxide synthase and, effect of substrate manipulation of the nitric oxide pathway in mouse ovarian follicles. HUMAN REPRODUCTION, 19 (1). pp. 30-40. ISSN 0268-1161

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Abstract

BACKGROUND: Nitric oxide (NO) is a cell messenger with multiple actions in different biological systems, implicated in the control of follicle and oocyte function. NO is formed from L-arginine by isoforms of nitric oxide synthase (NOS) via N-G-hydroxy-L-arginine, with L-citrulline as a byproduct. This study aimed to show how modulation of NO by manipulating NOS substrates would affect mouse follicle growth and ovulation in vitro, where vascular effects of NO are attenuated. METHODS: Immunohistochemistry [endothelial (eNOS) and inducible (iNOS)] and in situ hybridization (iNOS) were applied on mouse ovaries. Cultured follicles were also stained for iNOS by immunohistochemistry. For follicles cultured in the presence or absence of L-arginine, the ability of L-citrulline or N-G-hydroxy-L-arginine to substitute for L-arginine was assessed in terms of follicle growth and ovulation. RESULTS: iNOS and eNOS were localized in oocytes and theca, with some staining in granulosa. iNOS mRNA occurred predominantly in granulosa and oocyte. Omission Of L-arginine significantly reduced follicle survival and ovulation. Partial compensation for L-arginine withdrawal was achieved with L-citrulline and N-G-hydroxy-L-arginine. Specific abnormalities of follicle growth were noted. CONCLUSIONS: NOS is present in mouse follicles, and its action is necessary at a local level for normal follicle development in vitro. Reduced growth, persistent basement membranes and reduced ovulation were associated with in vitro disruption of NO.

Item Type:	Journal Article
Subjects:	R Medicine > RG Gynecology and obstetrics
Journal or Publication Title:	HUMAN REPRODUCTION
Publisher:	OXFORD UNIV PRESS
ISSN:	0268-1161
Official Date:	January 2004
Volume:	19
Number:	1
Number of Pages:	11
Page Range:	pp. 30-40
Identification Number:	10.1093/humrep/deh032
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/8920

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