Brown, Richard Francis (2016) Investigating the evolutionary origins and cell biology of Negativicutes. PhD thesis, University of Warwick.

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Abstract

The Negativicutes are a class of bacteria within the predominantly Grampositive phylum Firmicutes that possess a diderm Gram-negative cell envelope along with lipopolysaccharides. Remarkably, some members of this class of Gram-negative bacteria can form endospores. The evolutionary origins of this enigmatic class were investigated through bioinformatics analyses, which defined and characterised their Gram-negative and Gram-positive toolkits, while analyses of Gram-negative signature proteins provided insights into the origins of the Gram-negative toolkit. The genome of Sporomusa sphaeroides, a sporulating species from the Negativicutes, was sequenced and annotated for the first time. In addition, the transcriptional landscapes of Veillonella parvula and Sporomusa sphaeroides during logarithmic and stationary phase growth were investigated using RNA-Seq, expanding the repertoire of known genome features, including non-coding RNAs within these species.

Item Type:	Thesis or Dissertation (PhD)
Subjects:	Q Science > QH Natural history
Library of Congress Subject Headings (LCSH):	Phylogeny -- Molecular aspects, Gram's stain, Stains and staining (Microscopy), Gram-negative bacteria, Cytology
Official Date:	October 2016
Dates:	Date Event October 2016 Submitted
Institution:	University of Warwick
Theses Department:	Warwick Medical School
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Pallen, Mark J. ; Denham, Emma
Format of File:	pdf
Extent:	xvii, 214 leaves : illustrations, charts
Language:	eng

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