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Image based modeling of bleb site selection

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Collier, Sharon, Paschke, Peggy, Kay, Robert R. and Bretschneider, Till (2017) Image based modeling of bleb site selection. Scientific Reports, 7 . 6692. doi:10.1038/s41598-017-06875-9

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Official URL: http://dx.doi.org/10.1038/s41598-017-06875-9

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Abstract

Cells often employ fast, pressure-driven blebs to move through tissues or against mechanical resistance, but how bleb sites are selected and directed to the cell front remains an open question. Previously, we found that chemotaxing Dictyostelium cells preferentially bleb from concave regions, where membrane tension facilitates membrane-cortex detachment. Now, through a novel modeling approach based on actual cell contours, we use cell geometry to predict where blebs will form in migrating cells. We find that cell geometry alone, and by implication, physical forces in the membrane, is sufficient to predict the location of blebs in rounded cells moving in a highly resistive environment. The model is less successful with more polarized cells moving against less resistance, but can be greatly improved by positing a front-to-back gradient in membrane-cortex adhesion. In accord with this prediction, we find that Talin, which links membrane and cortex, forms such a front-to-back gradient. Thus our model provides a means of dissecting out the role of physical forces in controlling where blebs form, and shows that in certain circumstances they could be the major determining factor.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science > Computer Science
Faculty of Science > Molecular Organisation and Assembly in Cells (MOAC)
Library of Congress Subject Headings (LCSH): Cytology, Cell membranes, Dictyostelium
Journal or Publication Title: Scientific Reports
Publisher: Nature Publishing Group
ISSN: 2045-2322
Official Date: 27 July 2017
Dates:
DateEvent
27 July 2017Published
20 June 2017Accepted
Volume: 7
Article Number: 6692
DOI: 10.1038/s41598-017-06875-9
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
U105115237[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
EP/F500378/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
BB/M01150X/1 [BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
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