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Biophysical characterization of a protein for structure comparison : methods for identifying insulin structural changes

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Sklepari, Meropi, Rodger, Alison, Reason, A., Jamshidi, Shirin, Prokes, Ivan and Blindauer, Claudia A. (2016) Biophysical characterization of a protein for structure comparison : methods for identifying insulin structural changes. Analytical Methods, 8 (41). pp. 7460-7471. doi:10.1039/c6ay01573e

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Official URL: https://doi.org/10.1039/C6AY01573E

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Abstract

Although protein structure has been studied for many decades it remains the case that we cannot state with confidence whether two samples have the same molecular structure, particularly in solution. The increasing number of biosimilar biopharmaceutical drugs that are being tested means this is not an academic exercise. In this work we consider how four well-established techniques: dynamic light scattering (DLS), circular dichroism (CD), nuclear magnetic resonance spectroscopy (NMR), and molecular modelling can be combined to provide information about the supposedly well-understood protein insulin. A goal of this work was to establish a systematic means of detecting differences between insulin samples as a function of pH, temperature, and the presence or absence of zinc, all of which are known to change the oligomerisation state and to affect molecular structure. We used the recently developed Secondary Structure Neural Network (SSNN) circular dichroism algorithm to facilitate analysis of the CD spectra.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Science > Chemistry
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Proteins -- Molecular aspects, Insulin
Journal or Publication Title: Analytical Methods
Publisher: Royal Society of Chemistry
ISSN: 1759-9660
Official Date: 30 September 2016
Dates:
DateEvent
30 September 2016Available
29 September 2016Accepted
Volume: 8
Number: 41
Page Range: pp. 7460-7471
DOI: 10.1039/c6ay01573e
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
UNSPECIFIEDSeventh Framework Programmehttp://dx.doi.org/10.13039/100011102

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