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Roles of dicer-like proteins 2 and 4 in intra- and intercellular antiviral silencing
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Qin, Cheng, Li, Bin, Fan, Yaya, Zhang, Xian, Yu, Zhiming, Ryabov, Eugene, Zhao, Mei, Wang, Hui, Shi, Nongnong, Zhang, Pengcheng, Jackson, Stephen D. , Tör, Mahmut, Cheng, Qi, Liu, Yule, Gallusci, Philippe and Hong, Yiguo (2017) Roles of dicer-like proteins 2 and 4 in intra- and intercellular antiviral silencing. Plant Physiology, 174 (2). pp. 1067-1081. doi:10.1104/pp.17.00475 ISSN 0032-0889.
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Official URL: http://dx.doi.org/10.1104/pp.17.00475
Abstract
RNA silencing is an innate antiviral mechanism conserved in organisms across kingdoms. Such a cellular defense involves DICER or DICER-LIKEs (DCLs) that process plant virus RNAs into viral small interfering RNAs (vsiRNAs). Plants encode four DCLs that play diverse roles in cell-autonomous intracellular virus-induced RNA silencing (known as VIGS) against viral invasion. VIGS can spread between cells. However, the genetic basis and involvement of vsiRNAs in non-cell-autonomous intercellular VIGS remains poorly understood. Using GFP as a reporter gene together with a suite of DCL RNAi transgenic lines, here we show that despite the well-established activities of DCLs in intracellular VIGS and vsiRNA biogenesis, DCL4 acts to inhibit intercellular VIGS whereas DCL2 is required (likely along with DCL2-processed/dependent vsiRNAs and their precursor RNAs) for efficient intercellular VIGS trafficking from epidermal to adjacent cells. DCL4 imposed an epistatic effect on DCL2 to impede cell-to-cell spread of VIGS. Our results reveal previously unknown functions for DCL2 and DCL4 that may form a dual defensive frontline for intra- and intercellular silencing to double-protect cells from virus infection in Nicotiana benthamiana.
Item Type: | Journal Article | ||||||
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Subjects: | Q Science > QR Microbiology | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Library of Congress Subject Headings (LCSH): | Plant viruses -- Genetic aspects, RNA viruses | ||||||
Journal or Publication Title: | Plant Physiology | ||||||
Publisher: | American Society of Plant Biologists | ||||||
ISSN: | 0032-0889 | ||||||
Official Date: | 5 June 2017 | ||||||
Dates: |
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Volume: | 174 | ||||||
Number: | 2 | ||||||
Page Range: | pp. 1067-1081 | ||||||
DOI: | 10.1104/pp.17.00475 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
Date of first compliant deposit: | 24 July 2017 | ||||||
Funder: | Guo jia zi ran ke xue ji jin wei yuan hui (China) [National Natural Science Foundation of China] (NSFC), China. Nong ye bu [Ministry of Agriculture], Hangzhou da xue, Hangzhou Shi (China), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Zhōngguó zhèjiāng shěng zìrán kēxué jījīn (China) [Zhejiang Provincial Natural Science Foundation of China] (ZPNSFC) | ||||||
Grant number: | 31370180, 31500251, 31201490, 31200913 (NSFC), 016ZX08009001-004 (China. Nong ye bu [Ministry of Agriculture]), 201108 (Hangzhou da xue), 20131028 (Hangzhou Shi (China)), BB/K021079/1 (BBSRC), LY15C14006, LY14C010005 (ZPNSFC) |
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