The Library
Visualizing antimicrobials in bacterial biofilms : three-dimensional biochemical imaging using TOF-SIMS
Tools
Davies, Sarah K., Fearn, Sarah, Allsopp, Luke P., Harrison, Freya, Ware, Ecaterina, Diggle, Stephen P., Filloux, Alain, McPhail, David S. and Bundy, Jacob G. (2017) Visualizing antimicrobials in bacterial biofilms : three-dimensional biochemical imaging using TOF-SIMS. mSphere, 2 (4). 2:e00211-17. doi:10.1128/mSphere.00211-17 ISSN 2379-5042.
|
PDF
WRAP-visualizing-antimicrobials-bacterial-biofilms-Harrison-2017.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (4Mb) | Preview |
Official URL: http://dx.doi.org/10.1128/mSphere.00211-17
Abstract
Bacterial biofilms are groups of bacteria that exist within a self-produced extracellular matrix, adhering to each other and usually to a surface. They grow on medical equipment and inserts such as catheters and are responsible for many persistent infections throughout the body, as they can have high resistance to many antimicrobials. Pseudomonas aeruginosa is an opportunistic pathogen that can cause both acute and chronic infections and is used as a model for research into biofilms. Direct biochemical methods of imaging of molecules in bacterial biofilms are of high value in gaining a better understanding of the fundamental biology of biofilms and biochemical gradients within them. Time of flight–secondary-ion mass spectrometry (TOF-SIMS) is one approach, which combines relatively high spatial resolution and sensitivity and can perform depth profiling analysis. It has been used to analyze bacterial biofilms but has not yet been used to study the distribution of antimicrobials (including antibiotics and the antimicrobial metal gallium) within biofilms. Here we compared two methods of imaging of the interior structure of P. aeruginosa in biological samples using TOF-SIMS, looking at both antimicrobials and endogenous biochemicals: cryosectioning of tissue samples and depth profiling to give pseudo-three-dimensional (pseudo-3D) images. The sample types included both simple biofilms grown on glass slides and bacteria growing in tissues in an ex vivo pig lung model. The two techniques for the 3D imaging of biofilms are potentially valuable complementary tools for analyzing bacterial infection.
Item Type: | Journal Article | ||||||
---|---|---|---|---|---|---|---|
Subjects: | Q Science > QR Microbiology | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Library of Congress Subject Headings (LCSH): | Biofilms -- Imaging, Pseudomonas aeruginosa, Anti-infective agents | ||||||
Journal or Publication Title: | mSphere | ||||||
Publisher: | American Society for Microbiology | ||||||
ISSN: | 2379-5042 | ||||||
Official Date: | 19 July 2017 | ||||||
Dates: |
|
||||||
Volume: | 2 | ||||||
Number: | 4 | ||||||
Article Number: | 2:e00211-17 | ||||||
DOI: | 10.1128/mSphere.00211-17 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 26 July 2017 | ||||||
Date of first compliant Open Access: | 26 July 2017 | ||||||
Funder: | Wellcome Trust (London, England) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year