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Metallothionein from wild populations of the African catfish Clarias gariepinus : from sequence, protein expression and metal binding properties to transcriptional biomarker of metal pollution
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M'kandawire, Ethel, Mierek-Adamska, Agnieszka, Stürzenbaum, Stephen R., Choongo, Kennedy, Yabe, John, Mwase, Maxwell, Saasa, Ngonda and Blindauer, Claudia A. (2017) Metallothionein from wild populations of the African catfish Clarias gariepinus : from sequence, protein expression and metal binding properties to transcriptional biomarker of metal pollution. International Journal of Molecular Sciences, 18 (7). 1548. doi:10.3390/ijms18071548 ISSN 1422-0067.
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WRAP-metallothionein-wild-populations-african-catfish-clarias-gariepinus-M'kandawire-2017.pdf - Publisher's Proof Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2362Kb) | Preview |
Official URL: http://dx.doi.org/10.3390/ijms18071548
Abstract
Anthropogenic pollution with heavy metals is an on-going concern throughout the world, and methods to monitor release and impact of heavy metals are of high importance. With a view to probe its suitability as molecular biomarker of metal pollution, this study has determined a coding sequence for metallothionein of the African sharptooth catfish Clarias gariepinus. The gene product was recombinantly expressed in Escherichia coli in presence of Zn(II), Cd(II), or Cu, and characterised by Electrospray Ionisation Mass Spectrometry and elemental analysis. C. gariepinus MT displays typical features of fish MTs, including 20 conserved cysteines, and seven bound divalent cations (Zn(II) or Cd(II)) when saturated. Livers from wild C. gariepinus fish collected in all three seasons from four different sites on the Kafue River of Zambia were analysed for their metal contents and for MT expression levels by quantitative PCR. Significant correlations were found between Zn and Cu levels and MT expression in livers, with MT expression clearly highest at the most polluted site, Chililabombwe, which is situated in the Copperbelt region. Based on our findings, hepatic expression of MT from C. gariepinus may be further developed as a major molecular biomarker of heavy metal pollution resulting from mining activities in this region.
Item Type: | Journal Article | ||||||||||||
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Subjects: | Q Science > QL Zoology T Technology > TD Environmental technology. Sanitary engineering |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||||
SWORD Depositor: | Library Publications Router | ||||||||||||
Library of Congress Subject Headings (LCSH): | Kafue River (Zambia) -- Heavy metal content, Water -- Pollution -- Zambia, Metallothionein, Clarias gariepinus, Biochemical markers | ||||||||||||
Journal or Publication Title: | International Journal of Molecular Sciences | ||||||||||||
Publisher: | M D P I AG | ||||||||||||
ISSN: | 1422-0067 | ||||||||||||
Official Date: | 18 July 2017 | ||||||||||||
Dates: |
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Volume: | 18 | ||||||||||||
Number: | 7 | ||||||||||||
Article Number: | 1548 | ||||||||||||
DOI: | 10.3390/ijms18071548 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
RIOXX Funder/Project Grant: |
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