Multivalent conjugates of poly-gamma-D-glutamic acid from Bacillus licheniformis with antibody F(ab ') and glycopeptide Ligands
UNSPECIFIED. (2003) Multivalent conjugates of poly-gamma-D-glutamic acid from Bacillus licheniformis with antibody F(ab ') and glycopeptide Ligands. BIOCONJUGATE CHEMISTRY, 14 (6). pp. 1148-1155. ISSN 1043-1802Full text not available from this repository.
Official URL: http://dx.doi.org/10.1021/bc020019m
Poly-gamma-D-glutamic acid from Bacillus licheniformis is a water-soluble, nontoxic, nonimmunogenic exopolymer. Using synthetic linkers, the alpha-carboxylate side chains of PGA were conjugated to an exposed thiol side chain of an antibody F(ab') fragment, Mc109F4. Analysis of the PGA-Mc109F4 conjugate by gel filtration HPLC revealed a mixture of multivalent conjugates. The PGA-Mc109F4 conjugate retained biological activity, but showed a lower binding affinity to target BCL3B3 cells than free Mc109F4 F(ab')2 by flow cytometry, and a lower efficacy for BCL3B3 growth inhibition than free Mc109F4 F(ab')(2). PGA was also conjugated with the free amino group of glycopeptide antibiotic vancomycin. The PGA-vancomycin conjugate showed slightly lower antibacterial activity than free vancomycin versus susceptible Bacillus subtilis, but slightly higher activity versus intrinsically resistant Leuconostoc mesenteroides.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry|
|Journal or Publication Title:||BIOCONJUGATE CHEMISTRY|
|Publisher:||AMER CHEMICAL SOC|
|Official Date:||November 2003|
|Number of Pages:||8|
|Page Range:||pp. 1148-1155|
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