UNSPECIFIED. (2003) Multivalent conjugates of poly-gamma-D-glutamic acid from Bacillus licheniformis with antibody F(ab ') and glycopeptide Ligands. BIOCONJUGATE CHEMISTRY, 14 (6). pp. 1148-1155. ISSN 1043-1802

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Abstract

Poly-gamma-D-glutamic acid from Bacillus licheniformis is a water-soluble, nontoxic, nonimmunogenic exopolymer. Using synthetic linkers, the alpha-carboxylate side chains of PGA were conjugated to an exposed thiol side chain of an antibody F(ab') fragment, Mc109F4. Analysis of the PGA-Mc109F4 conjugate by gel filtration HPLC revealed a mixture of multivalent conjugates. The PGA-Mc109F4 conjugate retained biological activity, but showed a lower binding affinity to target BCL3B3 cells than free Mc109F4 F(ab')2 by flow cytometry, and a lower efficacy for BCL3B3 growth inhibition than free Mc109F4 F(ab')(2). PGA was also conjugated with the free amino group of glycopeptide antibiotic vancomycin. The PGA-vancomycin conjugate showed slightly lower antibacterial activity than free vancomycin versus susceptible Bacillus subtilis, but slightly higher activity versus intrinsically resistant Leuconostoc mesenteroides.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry
Journal or Publication Title:	BIOCONJUGATE CHEMISTRY
Publisher:	AMER CHEMICAL SOC
ISSN:	1043-1802
Date:	November 2003
Volume:	14
Number:	6
Number of Pages:	8
Page Range:	pp. 1148-1155
Identification Number:	10.1021/bc020019m
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/9136

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