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Cytoskeletal variations in an asymmetric cell division support diversity in nematode sperm size and sex ratios
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Winter, Ethan S., Schwarz, Anna, Fabig, Gunar, Feldman, Jessica L., Pires-da Silva, André Francisco, Müller-Reichert, Thomas, Sadler, Penny L. and Shakes, Diane C. (2017) Cytoskeletal variations in an asymmetric cell division support diversity in nematode sperm size and sex ratios. Development , 144 (18). pp. 3253-3263. doi:10.1242/dev.153841 ISSN 0950-1991.
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Official URL: http://dx.doi.org/10.1242/dev.153841
Abstract
Asymmetric partitioning is an essential component of many developmental processes. As spermatogenesis concludes, sperm are streamlined by discarding unnecessary cellular into cellular wastebags called residual bodies (RBs). During nematode spermatogenesis, this asymmetric partitioning event occurs shortly after anaphase II, and both microtubules and actin partition into a central RB. Here we use fluorescence and transmission electron microscopy to elucidate and compare the intermediate steps of RB formation in C. elegans, Rhabditis sp. SB347 (recently named Auanema rhodensis) and related nematodes. In all cases, intact microtubules reorganize and move from centrosomal to non-centrosomal sites at the RB-sperm boundary while actin reorganizes through cortical ring expansion and clearance from the poles. However, in species with tiny spermatocytes, these cytoskeletal changes are restricted to one pole. Consequently, partitioning yields one functional sperm with the X-bearing chromosome complement and an RB with the other chromosome set. Unipolar partitioning may not require an unpaired X, since it also occurs in XX spermatocytes. Instead, constraints related to spermatocyte downsizing may have contributed to the evolution of a sperm cell equivalent to female polar bodies.
| Item Type: | Journal Article | ||||||
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| Subjects: | Q Science > QH Natural history Q Science > QL Zoology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
| Library of Congress Subject Headings (LCSH): | Nematodes -- Spermatozoa, Meiosis | ||||||
| Journal or Publication Title: | Development | ||||||
| Publisher: | The Company of Biologists Ltd. | ||||||
| ISSN: | 0950-1991 | ||||||
| Official Date: | 21 August 2017 | ||||||
| Dates: |
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| Volume: | 144 | ||||||
| Number: | 18 | ||||||
| Page Range: | pp. 3253-3263 | ||||||
| DOI: | 10.1242/dev.153841 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||
| Date of first compliant deposit: | 24 August 2017 | ||||||
| Date of first compliant Open Access: | 21 August 2018 | ||||||
| Funder: | National Institutes of Health (U.S.) (NIH), National Science Foundation (U.S.) (NSF), Deutsche Forschungsgemeinschaft (DFG), College of William and Mary | ||||||
| Grant number: | 1R155GM096309-0 and DP2GM119136-01 (NIH), IOS 1122101 (NSF), MU 1423/3-2 and 10-1 (DFG) |
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