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Characterization of protein–protein interfaces in large complexes by solid-state NMR solvent paramagnetic relaxation enhancements
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Öster, Carl, Kosol, Simone, Hartmüller, Christoph, Lamley, Jonathan M., Iuga, Dinu, Oss, Andres, Org, Mai-Liis, Vanatalu, Kalju, Samoson, Ago, Madl, Tobias and Lewandowski, Józef R. (2017) Characterization of protein–protein interfaces in large complexes by solid-state NMR solvent paramagnetic relaxation enhancements. Journal of the American Chemical Society, 139 (35). pp. 12165-12174. doi:10.1021/jacs.7b03875 ISSN 0002-7863.
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Official URL: http://pubs.acs.org/doi/10.1021/jacs.7b03875
Abstract
Solid-state NMR is becoming a viable alternative for obtaining information about structures and dynamics of large biomolecular complexes, including ones that are not accessible to other high-resolution biophysical techniques. In this context, methods for probing protein−protein interfaces at atomic resolution are highly desirable. Solvent paramagnetic relaxation enhancements (sPREs) proved to be a powerful method for probing protein−protein interfaces in large complexes in solution but have not been employed toward this goal in the solid state. We demonstrate that 1H and 15N relaxation-based sPREs provide a powerful tool for characterizing intermolecular interactions in large assemblies in the solid state. We present approaches for measuring sPREs in practically the entire range of magic angle spinning frequencies used for biomolecular studies and discuss their benefits and limitations. We validate the approach on crystalline GB1, with our experimental results in good agreement with theoretical predictions. Finally, we use sPREs to characterize protein−protein interfaces in the GB1 complex with immunoglobulin G (IgG). Our results suggest the potential existence of an additional binding site and provide new insights into GB1:IgG complex structure that amend and revise the current model available from studies with IgG fragments. We demonstrate sPREs as a practical, widely applicable, robust, and very sensitive technique for determining intermolecular interaction interfaces in large biomolecular complexes in the solid state.
| Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Subjects: | Q Science > QD Chemistry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Protein-protein interactions, Nuclear magnetic resonance spectroscopy | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Journal or Publication Title: | Journal of the American Chemical Society | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Publisher: | American Chemical Society | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ISSN: | 0002-7863 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Official Date: | 6 September 2017 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Volume: | 139 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Number: | 35 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Page Range: | pp. 12165-12174 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| DOI: | 10.1021/jacs.7b03875 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Date of first compliant deposit: | 25 August 2017 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Date of first compliant Open Access: | 29 August 2017 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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