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Subcellular localisation and interactions among rubber particle proteins from Hevea braziliensis
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Brown, Daniel, Feeney, Mistianne, Hamadi, Mathin, Lonoce, Chiara, Sajari, Roslinda, Di Cola, Alessandra and Frigerio, Lorenzo (2017) Subcellular localisation and interactions among rubber particle proteins from Hevea braziliensis. Journal of Experimental Botany, 68 (18). pp. 5045-5055. doi:10.1093/jxb/erx331 ISSN 0022-0957.
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Official URL: https://doi.org/10.1093/jxb/erx331
Abstract
Natural rubber (polyisoprene) from the rubber tree Hevea brasiliensis is synthesized by specialized cells called laticifers. It is not clear how rubber particles arise, although one hypothesis is that they derive from the endoplasmic reticulum (ER) membrane. Here we cloned the genes encoding four key proteins found in association with rubber particles and studied their intracellular localization by transient expression in Nicotiana benthamiana leaves. We show that, while the cis-prenyltransferase (CPT), responsible for the synthesis of long polyisoprene chains, is a soluble, cytosolic protein, other rubber particle proteins such as rubber elongation factor (REF), small rubber particle protein (SRPP) and Hevea rubber transferase 1-REF bridging protein (HRBP) are associated with the endoplasmic reticulum (ER). We also show that SRPP can recruit CPT to the ER and that interaction of CPT with HRBP leads to both proteins relocating to the plasma membrane. We discuss these results in the context of the biogenesis of rubber particles.
Item Type: | Journal Article | |||||||||
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Subjects: | S Agriculture > SB Plant culture | |||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
Library of Congress Subject Headings (LCSH): | Rubber -- Synthesis, Hevea, Life -- Origin, Endoplasmic reticulum, Proteins -- Physiological transport, Biosynthesis | |||||||||
Journal or Publication Title: | Journal of Experimental Botany | |||||||||
Publisher: | OUP | |||||||||
ISSN: | 0022-0957 | |||||||||
Official Date: | 2 November 2017 | |||||||||
Dates: |
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Volume: | 68 | |||||||||
Number: | 18 | |||||||||
Page Range: | pp. 5045-5055 | |||||||||
DOI: | 10.1093/jxb/erx331 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||
Date of first compliant deposit: | 25 August 2017 | |||||||||
Date of first compliant Open Access: | 17 November 2017 | |||||||||
RIOXX Funder/Project Grant: |
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