Niehaus, Anne Marie S., Vlachos, Dionisios G., Edwards, Jeremy S., Plechac, Petr and Tribe, Roger. (2008) Microscopic simulation of membrane molecule diffusion on corralled membrane surfaces. Biophysical Journal, Vol.94 (No.5). pp. 1551-1564. ISSN 0006-3495

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Abstract

The current understanding of how receptors diffuse and cluster in the plasma membrane is limited. Data from single-particle tracking and laser tweezer experiments have suggested that membrane molecule diffusion is affected by the presence of barriers dividing the membrane into corrals. Here, we have developed a stochastic spatial model to simulate the effect of corrals on the diffusion of molecules in the plasma membrane. The results of this simulation confirm that a fence barrier (the ratio of the transition probability for diffusion across a boundary to that within a corral) on the order of 103–104 recreates the experimentally measured difference in diffusivity between artificial and natural plasma membranes. An expression for the macroscopic diffusivity of receptors on corralled membranes is derived to analyze the effects of the corral parameters on diffusion rate. We also examine whether the lattice model is an appropriate description of the plasma membrane and look at three different sets of boundary conditions that describe diffusion over the barriers and whether diffusion events on the plasma membrane may occur with a physically relevant length scale. Finally, we show that to observe anomalous (two-timescale) diffusion, one needs high temporal (microsecond) resolution along with sufficiently long (more than milliseconds) trajectories.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Science > Mathematics
Library of Congress Subject Headings (LCSH):	Cell membranes, Lattice field theory, Diffusion processes, Monte Carlo method, Cell receptors
Journal or Publication Title:	Biophysical Journal
Publisher:	Biophysical Society
ISSN:	0006-3495
Date:	March 2008
Volume:	Vol.94
Number:	No.5
Page Range:	pp. 1551-1564
Identification Number:	10.1529/biophysj.107.106484
Status:	Peer Reviewed
Access rights to Published version:	Open Access
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URI:	http://wrap.warwick.ac.uk/id/eprint/919

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