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Intracellular accumulation of methylglyoxal by glyoxalase 1 knock down alters collagen homoeostasis in L6 myoblasts

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Stratmann, Bernd, Goldstein, Bernhard, Thornalley, Paul J., Rabbani, Naila and Tschoepe, Diethelm (2017) Intracellular accumulation of methylglyoxal by glyoxalase 1 knock down alters collagen homoeostasis in L6 myoblasts. International Journal of Molecular Sciences, 18 (3). 480. doi:10.3390/ijms18030480

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Official URL: http://dx.doi.org/10.3390/ijms18030480

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Abstract

Hyperglycemia results in accumulation of the reactive dicarbonyl methylglyoxal (MG). Methylglyoxal is detoxified by the glyoxalase system (glyoxalase 1 and 2). The influence of glyoxalase 1 knockdown on expression of collagens 1, 3, 4, and 5 in L6 myoblasts under hyperglycemic conditions was investigated. Increased biosynthesis of collagens 1, 3, 4, and 5 was detected at mRNA-level following knockdown of glyoxalase 1 (GLO1). At the protein level a significant elevation of the concentration of collagen 1 and 4 was shown, whereas no increase of collagen 5 and a non-significant increase in collagen 3 were detectable. These results could partially explain MG-induced changes in the extracellular matrix (ECM) which account for increased fibrosis and impaired function in myocytes. The mechanisms by which reactive glucose metabolites influence ECM composition deserve further investigation. View Full-Text

Item Type: Journal Article
Divisions: Faculty of Medicine > Warwick Medical School
Journal or Publication Title: International Journal of Molecular Sciences
Publisher: M D P I AG
ISSN: 1422-0067
Official Date: 23 February 2017
Dates:
DateEvent
23 February 2017Published
17 February 2017Accepted
Volume: 18
Number: 3
Article Number: 480
DOI: 10.3390/ijms18030480
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access

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