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Extending the biocatalytic scope of regiocomplementary flavin-dependent halogenase enzymes
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Shepherd, Sarah A., Karthikeyan, Chinnan, Latham, Jonathan, Struck, Anna-Winona, Thompson, Mark L., Menon, Binuraj R. K., Styles, Matthew Q., Levy, Colin, Leys, David and Micklefield, Jason (2015) Extending the biocatalytic scope of regiocomplementary flavin-dependent halogenase enzymes. Chemical Science, 6 (6). pp. 3454-3460. doi:10.1039/C5SC00913H ISSN 2041-6520.
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Official URL: http://dx.doi.org/10.1039/C5SC00913H
Abstract
Flavin-dependent halogenases are potentially valuable biocatalysts for the regioselective halogenation of aromatic compounds. These enzymes, utilising benign inorganic halides, offer potential advantages over traditional non-enzymatic halogenation chemistry that often lacks regiocontrol and requires deleterious reagents. Here we extend the biocatalytic repertoire of the tryptophan halogenases, demonstrating how these enzymes can halogenate a range of alternative aryl substrates. Using structure guided mutagenesis we also show that it is possible to alter the regioselectivity as well as increase the activity of the halogenases with non-native substrates including anthranilic acid; an important intermediate in the synthesis and biosynthesis of pharmaceuticals and other valuable products.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Chemical Science | ||||
Publisher: | Royal Society of Chemistry | ||||
ISSN: | 2041-6520 | ||||
Official Date: | 2015 | ||||
Dates: |
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Volume: | 6 | ||||
Number: | 6 | ||||
Page Range: | pp. 3454-3460 | ||||
DOI: | 10.1039/C5SC00913H | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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