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Production of embryos from microspore cultures of Portuguese tronchuda cabbage landraces
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Vicente, Joana G. and Dias, J. S. (1996) Production of embryos from microspore cultures of Portuguese tronchuda cabbage landraces. Acta Horticulturae (407). pp. 219-226. doi:doi:10.17660/ActaHortic.1996.407.26 ISSN 0567-7572.
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Official URL: http://dx.doi.org/10.17660/ActaHortic.1996.407.26
Abstract
Twelve accessions of tronchuda cabbage landraces were tested for their ability to produce embryos through microspore culture in NLN-13 medium. A sample from all the isolations was stained with DAPI for determination of microspore developmental stage. The relationship between the microspore developmental stage and the production of microspore-derived embryos was evaluated.
Embryos were obtained from all the accessions. Considerable variation was observed between isolations with different developmental stage of microspores, accessions and plants within the same accession. The best embryogenic responses occurred in microspore populations with approximately 10 to 89% of binucleate pollen. The highest embryo yield was obtained with one of the ‘Couve de Valhascos’ accessions (0.41 embryos / 1 000 microspores) and the lowest yields with the accessions of ‘Couve Portuguesa’ (less than 0.07 embryos / 1 000 microspores).
Item Type: | Journal Article | ||||
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Subjects: | S Agriculture > SB Plant culture | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Cabbage -- Embryos , Cole crops | ||||
Journal or Publication Title: | Acta Horticulturae | ||||
Publisher: | International Society for Horticultural Science | ||||
ISSN: | 0567-7572 | ||||
Official Date: | 1996 | ||||
Dates: |
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Number: | 407 | ||||
Page Range: | pp. 219-226 | ||||
DOI: | doi:10.17660/ActaHortic.1996.407.26 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 5 October 2017 | ||||
Date of first compliant Open Access: | 6 October 2017 | ||||
Funder: | Junta nacional de investigação cientifica e tecnológica (Portugal) | ||||
Grant number: | AGR/1483/92 | ||||
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