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Enhanced quality factor label-free biosensing with micro-cantilevers integrated into microfluidic systems
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Kartanas, Tadas, Ostanin, Victor , Challa, Pavan Kumar, Daly, Ronan, Charmet, Jérôme and Knowles, Tuomas P. J. (2017) Enhanced quality factor label-free biosensing with micro-cantilevers integrated into microfluidic systems. Analytical Chemistry, 89 (22). pp. 11929-11936. doi:10.1021/acs.analchem.7b01174 ISSN 0003-2700.
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WRAP-enhanced-quality-factor-label-free-biosensing-micro-cantilevers-integrated-microfluidic-systems-Charmet-2017.pdf - Accepted Version - Requires a PDF viewer. Download (1770Kb) | Preview |
Official URL: http://doi.org/10.1021/acs.analchem.7b01174
Abstract
Microelectromechanical systems (MEMS) have enabled the development of a new generation of sensor platforms. Acoustic sensor operation in liquid, the native environment of biomolecules, causes, however, significant degradation of sensing performance due to viscous drag and relies on the availability of capture molecules to bind analytes of interest to the sensor surface. Here we describe a strategy to interface MEMS sensors with microfluidic platforms through an aerosol spray. Our sensing platform comprises a microfluidic spray nozzle and a micro-cantilever array operated in dynamic mode within a closed loop oscillator. A solution containing the analyte is sprayed uniformly through pico-litre droplets onto the micro-cantilever surface; the micron-scale drops evaporate rapidly and leave the solutes behind, adding to the mass of the cantilever. This sensing scheme results in a 50-fold increase in the quality factor compared to operation in liquid, yet allows the analytes to be introduced into the sensing system from a solution phase. It achieves a 370 femtogram limit of detection and we demonstrate quantitative label-free analysis of inorganic salts and model proteins. These results demonstrate that the standard resolution limits of cantilever sensing in dynamic mode can be overcome with the integration of spray microfluidics with MEMS.
Item Type: | Journal Article | ||||||
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Subjects: | T Technology > TK Electrical engineering. Electronics Nuclear engineering | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Engineering > WMG (Formerly the Warwick Manufacturing Group) | ||||||
Library of Congress Subject Headings (LCSH): | Microelectromechanical systems, Microfluidics, Biomolecules | ||||||
Journal or Publication Title: | Analytical Chemistry | ||||||
Publisher: | American Chemical Society | ||||||
ISSN: | 0003-2700 | ||||||
Official Date: | 6 October 2017 | ||||||
Dates: |
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Volume: | 89 | ||||||
Number: | 22 | ||||||
Page Range: | pp. 11929-11936 | ||||||
DOI: | 10.1021/acs.analchem.7b01174 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Reuse Statement (publisher, data, author rights): | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://doi.org/10.1021/acs.analchem.7b01174 | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
Date of first compliant deposit: | 9 October 2017 | ||||||
Date of first compliant Open Access: | 6 October 2018 | ||||||
Funder: | Seventh Framework Programme (European Commission) (FP7), European Research Council (ERC), Engineering and Physical Sciences Research Council (EPSRC), Frances and Augustus Newman Foundation, University of Cambridge. Nanotechnologies Doctoral Training Centre (NanoDTC) | ||||||
Grant number: | PhysProt agreement no 337969 (ERC), EP/L015978/1 (EPSRC) | ||||||
Open Access Version: |
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