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Polyproline is a minimal antifreeze protein mimetic and enhances the cryopreservation of cell monolayers

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Graham, Ben, Bailey, Trisha L., Healey, Joseph R. J., Marcellini, Moreno, Deville, Sylvain and Gibson, Matthew I. (2017) Polyproline is a minimal antifreeze protein mimetic and enhances the cryopreservation of cell monolayers. Angewandte Chemie International Edition, 56 (50). pp. 15941-15944. doi:10.1002/anie.201706703

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Official URL: http://dx.doi.org/10.1002/anie.201706703

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Abstract

Tissue engineering, gene therapy, drug screening and emerging regenerative medicine therapies are fundamentally reliant on high-quality adherent cell culture, but current methods to cryopreserve cells in this format can give low cell yields and requires large volumes of solvent 'antifreezes'. Herein we report polyproline is a minimum (bio)synthetic mimic of antifreeze proteins, which is accessible by solution, solid phase and recombinant methods. We demonstrate that polyproline has ice recrystallization inhibition activity linked to its amphipathic helix and that it enhances the DMSO- cryopreservation of adherent cell lines. Polyproline may be a versatile additive in the emerging field of macromolecular cryoprotectants.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
R Medicine > R Medicine (General)
Divisions: Faculty of Science > Chemistry
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Biomedical materials., Surface chemistry., Cryopreservation of organs, tissues, etc. -- Methodology., Polymers., Ice crystals -- Growth.
Journal or Publication Title: Angewandte Chemie International Edition
Publisher: Wiley - V C H Verlag GmbH & Co. KGaA
ISSN: 1433-7851
Official Date: 11 December 2017
Dates:
DateEvent
11 December 2017Published
18 October 2017Available
18 October 2017Accepted
Date of first compliant deposit: 23 October 2017
Volume: 56
Number: 50
Page Range: pp. 15941-15944
DOI: 10.1002/anie.201706703
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
CRYO-MAT638661,278004FreeCoH2020 European Research Councilhttp://dx.doi.org/10.13039/100010663
BB/F011199/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
MOAC DTCEP/F500378/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
UNSPECIFIED[RS] Royal Societyhttp://dx.doi.org/10.13039/501100000288

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