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Engineered hydrogen-bonded glycopolymer capsules and their interactions with antigen presenting cells
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Kempe, Kristian, Xiang, Sue D., Wilson, Paul, Rahim, Md. Arifur, Ju, Yi, Whittaker, Michael R., Haddleton, David M., Plebanski, Magdalena, Caruso, Frank and Davis, Thomas P. (2017) Engineered hydrogen-bonded glycopolymer capsules and their interactions with antigen presenting cells. ACS Applied Materials & Interfaces, 9 (7). pp. 6444-6452. doi:10.1021/acsami.6b15459
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Official URL: https://doi.org/10.1021/acsami.6b15459
Abstract
Hollow glycopolymer microcapsules were fabricated by hydrogen-bonded layer-by-layer (LbL) assembly, and their interactions with a set of antigen presenting cells (APCs), including dendritic cells (DCs), macrophages (MACs), and myeloid derived suppressor cells (MDSCs), were investigated. The glycopolymers were obtained by cascade postpolymerization modifications of poly(oligo(2-ethyl-2-oxazoline methacrylate)-stat-glycidyl methacrylate) involving the modification of the glycidyl groups with propargylamine and the subsequent attachment of mannose azide by copper(I)-catalyzed azide–alkyne cycloaddition (CuAAC). Multilayer assembly of the hydrogen-bonding pair (glycopolymer/poly(methacrylic acid) (PMA)) onto planar and particulate supports (SiO2 particles, d = 1.16 μm) yielded stable glycopolymer films upon cross-linking by CuAAC. The silica (SiO2) particle templates were removed yielding hollow monodisperse capsules, as demonstrated by fluorescence and scanning electron microscopy. Cellular uptake studies using flow cytometry revealed the preferential uptake of the capsules by DCs when compared to MACs or MDSCs. Mannosylated capsules showed a cytokine independent cis-upregulation of CD80 specifically on DCs and a trans-downregulation of PDL-1 on MDSCs. Thus, the glycopolymer capsules may have potential as vaccine carriers, as they are able to upregulate costimulatory molecules for immune cell stimulation on DCs and at the same time downregulate immune inhibitory receptors on suppressor APC such as MDSCs.
| Item Type: | Journal Article | ||||||||||||||||||||||||
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| Subjects: | R Medicine > RS Pharmacy and materia medica T Technology > TP Chemical technology |
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| Divisions: | Faculty of Science > Chemistry | ||||||||||||||||||||||||
| SWORD Depositor: | Library Publications Router | ||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Synthetic products, Drug delivery systems | ||||||||||||||||||||||||
| Journal or Publication Title: | ACS Applied Materials & Interfaces | ||||||||||||||||||||||||
| Publisher: | American Chemical Society (ACS) | ||||||||||||||||||||||||
| ISSN: | 1944-8252 | ||||||||||||||||||||||||
| Official Date: | 22 February 2017 | ||||||||||||||||||||||||
| Dates: |
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| Volume: | 9 | ||||||||||||||||||||||||
| Number: | 7 | ||||||||||||||||||||||||
| Page Range: | pp. 6444-6452 | ||||||||||||||||||||||||
| DOI: | 10.1021/acsami.6b15459 | ||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||
| Publisher Statement: | “This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials & Interfaces, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].” | ||||||||||||||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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