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Engineered hydrogen-bonded glycopolymer capsules and their interactions with antigen presenting cells

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Kempe, Kristian, Xiang, Sue D., Wilson, Paul, Rahim, Md. Arifur, Ju, Yi, Whittaker, Michael R., Haddleton, David M., Plebanski, Magdalena, Caruso, Frank and Davis, Thomas P. (2017) Engineered hydrogen-bonded glycopolymer capsules and their interactions with antigen presenting cells. ACS Applied Materials & Interfaces, 9 (7). pp. 6444-6452. doi:10.1021/acsami.6b15459

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Official URL: https://doi.org/10.1021/acsami.6b15459

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Abstract

Hollow glycopolymer microcapsules were fabricated by hydrogen-bonded layer-by-layer (LbL) assembly, and their interactions with a set of antigen presenting cells (APCs), including dendritic cells (DCs), macrophages (MACs), and myeloid derived suppressor cells (MDSCs), were investigated. The glycopolymers were obtained by cascade postpolymerization modifications of poly(oligo(2-ethyl-2-oxazoline methacrylate)-stat-glycidyl methacrylate) involving the modification of the glycidyl groups with propargylamine and the subsequent attachment of mannose azide by copper(I)-catalyzed azide–alkyne cycloaddition (CuAAC). Multilayer assembly of the hydrogen-bonding pair (glycopolymer/poly(methacrylic acid) (PMA)) onto planar and particulate supports (SiO2 particles, d = 1.16 μm) yielded stable glycopolymer films upon cross-linking by CuAAC. The silica (SiO2) particle templates were removed yielding hollow monodisperse capsules, as demonstrated by fluorescence and scanning electron microscopy. Cellular uptake studies using flow cytometry revealed the preferential uptake of the capsules by DCs when compared to MACs or MDSCs. Mannosylated capsules showed a cytokine independent cis-upregulation of CD80 specifically on DCs and a trans-downregulation of PDL-1 on MDSCs. Thus, the glycopolymer capsules may have potential as vaccine carriers, as they are able to upregulate costimulatory molecules for immune cell stimulation on DCs and at the same time downregulate immune inhibitory receptors on suppressor APC such as MDSCs.

Item Type: Journal Article
Subjects: R Medicine > RS Pharmacy and materia medica
T Technology > TP Chemical technology
Divisions: Faculty of Science > Chemistry
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Synthetic products, Drug delivery systems
Journal or Publication Title: ACS Applied Materials & Interfaces
Publisher: American Chemical Society (ACS)
ISSN: 1944-8252
Official Date: 22 February 2017
Dates:
DateEvent
22 February 2017Published
10 February 2017Available
25 January 2017Accepted
Volume: 9
Number: 7
Page Range: pp. 6444-6452
DOI: 10.1021/acsami.6b15459
Status: Peer Reviewed
Publication Status: Published
Publisher Statement: “This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials & Interfaces, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].”
Access rights to Published version: Restricted or Subscription Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
CE140100036[ARC] Australian Research Councilhttp://dx.doi.org/10.13039/501100000923
ECF/2015-075Leverhulme Trusthttp://dx.doi.org/10.13039/501100000275
APP1109945National Health and Medical Research Councilhttp://dx.doi.org/10.13039/501100000925
APP1109945[ARC] Australian Research Councilhttp://dx.doi.org/10.13039/501100000923
APP1059409National Health and Medical Research Councilhttp://dx.doi.org/10.13039/501100000925
FL120100030[ARC] Australian Research Councilhttp://dx.doi.org/10.13039/501100000923
FL140100052[ARC] Australian Research Councilhttp://dx.doi.org/10.13039/501100000923
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