The Library
Long-term imaging of endoplasmic reticulum morphology in embryos during seed germination.
Tools
Dzimitrowicz, Natasha, Breeze, Emily and Frigerio, Lorenzo (2018) Long-term imaging of endoplasmic reticulum morphology in embryos during seed germination. In: Hawes, C. and Kriechbaumer, V., (eds.) The Plant Endoplasmic Reticulum. Methods in Molecular Biology, 1691 . Humana Press, New York, NY: Springer, pp. 67-74. ISBN 9781493973897
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: https://doi.org/10.1007/978-1-4939-7389-7_6
Abstract
Imaging plant embryos at the cellular level over time is technically challenging, since the embryo, once its protective seed coat is removed, must be kept viable and unstressed on a microscope slide for the duration of the experiment. Here we describe a procedure and suitable apparatus for the visualization, over several days, of changes in endoplasmic reticulum (ER) morphology associated with the process of germination in Arabidopsis thaliana seeds. Moreover, we also present a user-friendly image analysis tool which enables subtle perturbations in the ER network to be measured.
Item Type: | Book Item | ||||
---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
SWORD Depositor: | Library Publications Router | ||||
Series Name: | Methods in Molecular Biology | ||||
Publisher: | Springer | ||||
Place of Publication: | Humana Press, New York, NY | ||||
ISBN: | 9781493973897 | ||||
ISSN: | 1064-3745 | ||||
Book Title: | The Plant Endoplasmic Reticulum | ||||
Editor: | Hawes, C. and Kriechbaumer, V. | ||||
Official Date: | 2018 | ||||
Dates: |
|
||||
Volume: | 1691 | ||||
Page Range: | pp. 67-74 | ||||
Publication Status: | Published | ||||
Reuse Statement (publisher, data, author rights): | ** From PubMed via Jisc Publications Router. | ||||
Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |