UNSPECIFIED (2003) Diminished expression of an antiviral ribonuclease in response to pneumovirus infection in vivo. ANTIVIRAL RESEARCH, 59 (3). pp. 181-191. ISSN 0166-3542

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Abstract

The mouse eosinophil-associated ribonucleases (mEars) are species specific, divergent orthologs of the human antiviral RNase A ribonucleases, eosinophil-derived neurotoxin (RNase 2) and eosinophil cationic protein (RNase 3). We show here that mEar 2 is also an antiviral ribonuclease, as micromolar concentrations promote a similar tosixfold reduction in the infectivity of pneumonia virus of mice (PVM) for target respiratory epithelial cells in vitro. Although initially identified as a component of eosinophilic leukocytes, mEar 2 mRNA and protein were also detected in lung tissue accompanied by enzymatically active mEar 2 in bronchoalveolar lavage fluid (BALF). At t = 3 days post-inoculation with PVM (strain J3666), we observed the characteristic inflammatory response accompanied by diminished expression of total mEar mRNA and protein in lung tissue and a corresponding fivefold drop in ribonuclease activity in BALF No change in mEar expression was observed in response to infection with PVM strain 15, a replication-competent strain of PVM that does not elicit a cellular inflammatory response. However, mEar expression is not directly dependent on inflammation per se, as diminished expression of mEar mRNA and BAL ribonuclease activity were also observed in PVM-infected, inflammation-deficient, MIP-1alpha -/- mice. We propose that this mechanism may represent a novel virus-mediated evasion strategy, with a mechanism that is linked in some fashion to virus-specific pathogenicity. Published by Elsevier B.V.

Item Type:	Journal Article
Subjects:	R Medicine > RS Pharmacy and materia medica Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title:	ANTIVIRAL RESEARCH
Publisher:	ELSEVIER SCIENCE BV
ISSN:	0166-3542
Date:	August 2003
Volume:	59
Number:	3
Number of Pages:	11
Page Range:	pp. 181-191
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/9387

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