Diminished expression of an antiviral ribonuclease in response to pneumovirus infection in vivo
UNSPECIFIED. (2003) Diminished expression of an antiviral ribonuclease in response to pneumovirus infection in vivo. ANTIVIRAL RESEARCH, 59 (3). pp. 181-191. ISSN 0166-3542Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/S0166-3542(03)00111-6
The mouse eosinophil-associated ribonucleases (mEars) are species specific, divergent orthologs of the human antiviral RNase A ribonucleases, eosinophil-derived neurotoxin (RNase 2) and eosinophil cationic protein (RNase 3). We show here that mEar 2 is also an antiviral ribonuclease, as micromolar concentrations promote a similar tosixfold reduction in the infectivity of pneumonia virus of mice (PVM) for target respiratory epithelial cells in vitro. Although initially identified as a component of eosinophilic leukocytes, mEar 2 mRNA and protein were also detected in lung tissue accompanied by enzymatically active mEar 2 in bronchoalveolar lavage fluid (BALF). At t = 3 days post-inoculation with PVM (strain J3666), we observed the characteristic inflammatory response accompanied by diminished expression of total mEar mRNA and protein in lung tissue and a corresponding fivefold drop in ribonuclease activity in BALF No change in mEar expression was observed in response to infection with PVM strain 15, a replication-competent strain of PVM that does not elicit a cellular inflammatory response. However, mEar expression is not directly dependent on inflammation per se, as diminished expression of mEar mRNA and BAL ribonuclease activity were also observed in PVM-infected, inflammation-deficient, MIP-1alpha -/- mice. We propose that this mechanism may represent a novel virus-mediated evasion strategy, with a mechanism that is linked in some fashion to virus-specific pathogenicity. Published by Elsevier B.V.
|Item Type:||Journal Article|
|Subjects:||R Medicine > RS Pharmacy and materia medica
Q Science > QR Microbiology > QR355 Virology
|Journal or Publication Title:||ANTIVIRAL RESEARCH|
|Publisher:||ELSEVIER SCIENCE BV|
|Number of Pages:||11|
|Page Range:||pp. 181-191|
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