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Profiling histone modifications in synchronized floral tissues for quantitative resolution of chromatin and transcriptome dynamics
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Engelhorn, Julia, Wellmer, Frank and Carles, Cristel C. (2018) Profiling histone modifications in synchronized floral tissues for quantitative resolution of chromatin and transcriptome dynamics. In: Bemer, M. and Baroux, C., (eds.) Plant Chromatin Dynamics. Methods in Molecular Biology, 1675 . New York, NY: Humana Press, pp. 271-296. ISBN 9781493973187
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Official URL: https://doi.org/10.1007/978-1-4939-7318-7_16
Abstract
Covalent histone modifications and their effects on chromatin state and accessibility play a key role in the regulation of gene expression in eukaryotes. To gain insights into their functions during plant growth and development, the distribution of histone modifications can be analyzed at a genome-wide scale through chromatin immunoprecipitation assays followed by sequencing of the isolated genomic DNA. Here, we present a protocol for systematic analysis of the distribution and dynamic changes of selected histone modifications, during flower development in the model plant Arabidopsis thaliana. This protocol utilizes a previously established floral induction system to synchronize flower development, which allows the collection of sufficient plant material for analysis by genomic technologies. In this chapter, we describe how to use this system to study, from the same set of samples, chromatin and transcriptome dynamics during early stages of flower formation.
Item Type: | Book Item | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
SWORD Depositor: | Library Publications Router | ||||||
Series Name: | Methods in Molecular Biology | ||||||
Publisher: | Humana Press | ||||||
Place of Publication: | New York, NY | ||||||
ISBN: | 9781493973187 | ||||||
ISSN: | 1940-6029 | ||||||
Book Title: | Plant Chromatin Dynamics | ||||||
Editor: | Bemer, M. and Baroux, C. | ||||||
Official Date: | 2018 | ||||||
Dates: |
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Volume: | 1675 | ||||||
Page Range: | pp. 271-296 | ||||||
Publication Status: | Published | ||||||
Reuse Statement (publisher, data, author rights): | ** From PubMed via Jisc Publications Router. | ||||||
Access rights to Published version: | Restricted or Subscription Access |
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