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Evidence for post-transcriptional regulation of interleukin-5 by dexamethasone
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UNSPECIFIED (2003) Evidence for post-transcriptional regulation of interleukin-5 by dexamethasone. IMMUNOLOGY, 109 (4). pp. 527-535.
Research output not available from this repository, contact author.Abstract
Interleukin-5 (IL-5) is a T helper type 2 cytokine, which is implicated in the pathogenesis of eosinophilic diseases such as asthma. Both peripheral blood mononuclear cells (PBMC) and primary human T cells display similar patterns of IL-5 expression when stimulated with both phorbol-12-myristate 13-acetate and phytohaemagglutinin. The expression of IL-5 stimulated by these agents was shown to require de novo transcription and translation. However, although dexamethasone was a potent inhibitor of both IL-5 release and messenger RNA accumulation from PBMC and T cells, dexamethasone had no effect on the luciferase activity of a reporter construct under the control of an IL-5 promoter region transiently transfected into primary human T cells. Furthermore, dexamethasone appeared to decrease the stability of IL-5 messenger RNA and this effect was dependent upon de novo transcription. Taken together, the results presented here suggest that, whilst transcriptional processes predominantly regulate IL-5 release, the mechanism by which dexamethasone inhibits IL-5 is post-transcriptional.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QR Microbiology > QR180 Immunology | ||||
| Journal or Publication Title: | IMMUNOLOGY | ||||
| Publisher: | BLACKWELL PUBLISHING LTD | ||||
| ISSN: | 0019-2805 | ||||
| Official Date: | August 2003 | ||||
| Dates: |
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| Volume: | 109 | ||||
| Number: | 4 | ||||
| Number of Pages: | 9 | ||||
| Page Range: | pp. 527-535 | ||||
| Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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