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PCR-based genotyping of Helicobacter pylori of Gambian children and adults directly from biopsy specimens and bacterial cultures
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Secka, O., Antonio, Martin, Tapgun, M., Berg, D. E., Bottomley, C., Thomas, V., Walton, Robert T., Corrah, T., Adegbola, R. A. and Thomas, J. E. (2011) PCR-based genotyping of Helicobacter pylori of Gambian children and adults directly from biopsy specimens and bacterial cultures. Gut Pathogens, 3 (1). 5. ISSN 1757-4749.
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Official URL: https://doi.org/10.1186/1757-4749-3-5
Abstract
Background: Helicobacter pylori is an important agent of gastroduodenal disease in Africa and throughout the world. We sought to determine an optimum method for genotyping H. pylori strains from children and adults in The Gambia, West Africa. Results: Virulence genes were amplified in 127 of 190 cases tested (121 adults and 6 children); each of 60 bacterial cultures, and 116 from DNA extracted directly from biopsies. The proportion of biopsies that were cagA+, the ratio of vacAs1/s2, and vacAm1/m2, and the proportion of mixed strain populations in individual subjects changed with age. Strains lacking virulence cagA and vacA genes and with apparently homogeneous (one predominant strain) infections were more common among infants than adults. Conclusions: In order to detect the range of bacterial genotypes harbored by individual patients, direct PCR proved slightly superior to isolation of H. pylori by biopsy culture, but the techniques were complementary, and the combination of both culture and direct PCR produced the most complete picture. The seemingly higher virulence of strains from adult than infant infections in The Gambia merits further analysis.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Health Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Health Sciences > Population, Evidence & Technologies (PET) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Gut Pathogens | ||||||
Publisher: | BioMed Central Ltd. | ||||||
ISSN: | 1757-4749 | ||||||
Official Date: | 20 April 2011 | ||||||
Dates: |
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Volume: | 3 | ||||||
Number: | 1 | ||||||
Article Number: | 5 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Reuse Statement (publisher, data, author rights): | cited By 12 | ||||||
Access rights to Published version: | Open Access (Creative Commons) |
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