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Evolutionary conserved cysteines function as cis-acting regulators of Arabidopsis PIN-FORMED 2 distribution
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Retzer, Katarzyna, Lacek, Jozef, Skokan, Roman, Del Genio, Charo I., Vosolsobě, Stanislav, Laňková, Martina, Malínská, Kateřina, Konstantinova, Nataliia, Zažímalová, Eva, Napier, R. (Richard), Petrášek, Jan and Luschnig, Christian (2017) Evolutionary conserved cysteines function as cis-acting regulators of Arabidopsis PIN-FORMED 2 distribution. International Journal of Molecular Sciences, 18 (11). 2274. doi:10.3390/ijms18112274 ISSN 1422-0067.
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Official URL: http://dx.doi.org/10.3390/ijms18112274
Abstract
Coordination of plant development requires modulation of growth responses that are under control of the phytohormone auxin. PIN-FORMED plasma membrane proteins, involved in intercellular transport of the growth regulator, are key to the transmission of such auxin signals and subject to multilevel surveillance mechanisms, including reversible post-translational modifications. Apart from well-studied PIN protein modifications, namely phosphorylation and ubiquitylation, no further post-translational modifications have been described so far. Here, we focused on root-specific Arabidopsis PIN2 and explored functional implications of two evolutionary conserved cysteines, by a combination of in silico and molecular approaches. PIN2 sequence alignments and modeling predictions indicated that both cysteines are facing the cytoplasm and therefore would be accessible to redox status-controlled modifications. Notably, mutant pin2C−A alleles retained functionality, demonstrated by their ability to almost completely rescue defects of a pin2 null allele, whereas high resolution analysis of pin2C−A localization revealed increased intracellular accumulation, and altered protein distribution within plasma membrane micro-domains. The observed effects of cysteine replacements on root growth and PIN2 localization are consistent with a model in which redox status-dependent cysteine modifications participate in the regulation of PIN2 mobility, thereby fine-tuning polar auxin transport. View Full-Text
| Item Type: | Journal Article | ||||||||||||||||||||||||
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| Subjects: | Q Science > QK Botany | ||||||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Auxin -- Molecular aspects, Arabidopsis, Cysteine | ||||||||||||||||||||||||
| Journal or Publication Title: | International Journal of Molecular Sciences | ||||||||||||||||||||||||
| Publisher: | M D P I AG | ||||||||||||||||||||||||
| ISSN: | 1422-0067 | ||||||||||||||||||||||||
| Official Date: | 29 October 2017 | ||||||||||||||||||||||||
| Dates: |
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| Volume: | 18 | ||||||||||||||||||||||||
| Number: | 11 | ||||||||||||||||||||||||
| Article Number: | 2274 | ||||||||||||||||||||||||
| DOI: | 10.3390/ijms18112274 | ||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||
| Date of first compliant deposit: | 9 November 2017 | ||||||||||||||||||||||||
| Date of first compliant Open Access: | 9 November 2017 | ||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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