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CHC22 and CHC17 clathrins have distinct biochemical properties and display differential regulation and function

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Dannhauser, Philip N., Camus, Stéphane M., Sakamoto, Kazuho, Sadacca, L. Amanda, Torres, Jorge A., Camus, Marine D., Briant, Kit, Vassilopoulos, Stéphane, Rothnie, Alice, Smith, Corinne J. and Brodsky, Frances M. (2017) CHC22 and CHC17 clathrins have distinct biochemical properties and display differential regulation and function. Journal of Biological Chemistry, 292 . pp. 20834-20844. doi:10.1074/jbc.M117.816256

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Official URL: http://doi.org/10.1074/jbc.M117.816256

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Abstract

Clathrins are cytoplasmic proteins that play essential roles in endocytosis and other membrane traffic pathways. Upon recruitment to intracellular membranes, the canonical clathrin triskelion assembles into a polyhedral protein coat that facilitates vesicle formation and captures cargo molecules for transport. The triskelion is formed by trimerization of three clathrin heavy-chain subunits. Most vertebrates have two isoforms of clathrin heavy chains, CHC17 and CHC22, generating two clathrins with distinct cellular functions. CHC17 forms vesicles at the plasma membrane for receptor-mediated endocytosis and at the trans-Golgi network for organelle biogenesis. CHC22 plays a key role in intracellular targeting of the insulin-regulated glucose transporter 4 (GLUT4), accumulates at the site of GLUT4 sequestration during insulin resistance, and has also been implicated in neuronal development. Here, we demonstrate that CHC22 and CHC17 share morphological features, in that CHC22 forms a triskelion and latticed vesicle coats. However, cellular CHC22-coated vesicles were distinct from those formed by CHC17. The CHC22 coat was more stable to pH change and was not removed by the enzyme complex that disassembles the CHC17 coat. Moreover, the two clathrins were differentially recruited to membranes by adaptors, and CHC22 did not support vesicle formation or transferrin endocytosis at the plasma membrane in the presence or absence of CHC17. Our findings provide biochemical evidence for separate regulation and distinct functional niches for CHC17 and CHC22 in human cells. Furthermore, the greater stability of the CHC22 coat relative to the CHC17 coat may be relevant to its excessive accumulation with GLUT4 during insulin resistance. [Abstract copyright: Copyright © 2017, The American Society for Biochemistry and Molecular Biology.]

Item Type: Journal Article
Subjects: Q Science > QH Natural history
Divisions: Faculty of Science > Life Sciences (2010- )
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Cytoplasm, Endocytosis, Coated vesicles
Journal or Publication Title: Journal of Biological Chemistry
Publisher: American Society for Biochemistry and Molecular Biology
ISSN: 0021-9258
Official Date: 22 December 2017
Dates:
DateEvent
22 December 2017Published
2 November 2017Available
30 October 2017Accepted
Date of first compliant deposit: 22 December 2017
Volume: 292
Page Range: pp. 20834-20844
DOI: 10.1074/jbc.M117.816256
Status: Peer Reviewed
Publication Status: Published
Publisher Statement: ** From PubMed via Jisc Publications Router. ** History: received 05-09-2017; accepted 02-11-2017.
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
R56-DK083589National Institutes of Healthhttp://dx.doi.org/10.13039/100000002
P30-DK063720National Institutes of Healthhttp://dx.doi.org/10.13039/100000002
R01-DK095663National Institutes of Healthhttp://dx.doi.org/10.13039/100000002
107858/Z/15/ZWellcome Trusthttp://dx.doi.org/10.13039/100004440
UNSPECIFIEDAmerican Heart Associationhttp://dx.doi.org/10.13039/100000968
25460338Japan Society for the Promotion of Sciencehttp://dx.doi.org/10.13039/501100001691
UNSPECIFIEDRoyal Scientific Societyhttp://dx.doi.org/10.13039/501100007862
G0601125[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
Excellence Fellowship University College Londonhttp://dx.doi.org/10.13039/501100000765

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