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Engineering of biomolecules by bacteriophage directed evolution
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Brödel, Andreas K., Isalan, Mark and Jaramillo, Alfonso (2018) Engineering of biomolecules by bacteriophage directed evolution. Current Opinion in Biotechnology, 51 . pp. 32-38. doi:10.1016/j.copbio.2017.11.004 ISSN 0958-1669.
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Official URL: http://dx.doi.org/10.1016/j.copbio.2017.11.004
Abstract
Conventional in vivo directed evolution methods have primarily linked the biomolecule’s activity to bacterial cell growth. Recent developments instead rely on the conditional growth of bacteriophages (phages), viruses that infect and replicate within bacteria. Here we review recent phage-based selection systems for in vivo directed evolution. These approaches have been applied to evolve a wide range of proteins including transcription factors, polymerases, proteases, DNA-binding proteins, and protein-protein interactions. Advances in this field expand the possible applications of protein and RNA engineering. This will ultimately result in new biomolecules with tailor-made properties, as well as giving us a better understanding of basic evolutionary processes.
Item Type: | Journal Article | |||||||||||||||||||||
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Subjects: | Q Science > QH Natural history | |||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Biomechanics, Protein engineering, Biomolecules, Molecular evolution, Bacteriophages | |||||||||||||||||||||
Journal or Publication Title: | Current Opinion in Biotechnology | |||||||||||||||||||||
Publisher: | Elsevier Ltd. | |||||||||||||||||||||
ISSN: | 0958-1669 | |||||||||||||||||||||
Official Date: | June 2018 | |||||||||||||||||||||
Dates: |
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Volume: | 51 | |||||||||||||||||||||
Page Range: | pp. 32-38 | |||||||||||||||||||||
DOI: | 10.1016/j.copbio.2017.11.004 | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||||||||
Date of first compliant deposit: | 24 November 2017 | |||||||||||||||||||||
Date of first compliant Open Access: | 22 November 2018 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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