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Multimodal probes : superresolution and transmission electron microscopy imaging of mitochondria, and oxygen mapping of cells, using small-molecule Ir(III) luminescent complexes

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Shewring, Jonathan R., Cankut, Ahmet J., McKenzie, Luke K., Crowston, Bethany J., Botchway, Stanley W., Weinstein, Julia A., Edwards, Elizabeth and Ward, Michael D. (2017) Multimodal probes : superresolution and transmission electron microscopy imaging of mitochondria, and oxygen mapping of cells, using small-molecule Ir(III) luminescent complexes. Inorganic Chemistry, 56 (24). pp. 15259-15270. doi:10.1021/acs.inorgchem.7b02633

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Official URL: https://doi.org/10.1021/acs.inorgchem.7b02633

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Abstract

We describe an Ir(III)-based small-molecule, multimodal probe for use in both light and electron microscopy. The direct correlation of data between light- and electron-microscopy-based imaging to investigate cellular processes at the ultrastructure level is a current challenge, requiring both dyes that must be brightly emissive for luminescence imaging and scatter electrons to give contrast for electron microscopy, at a single working concentration suitable for both methods. Here we describe the use of Ir(III) complexes as probes that provide excellent image contrast and quality for both luminescence and electron microscopy imaging, at the same working concentration. Significant contrast enhancement of cellular mitochondria was observed in transmission electron microscopy imaging, with and without the use of typical contrast agents. The specificity for cellular mitochondria was also confirmed with MitoTracker using confocal and 3D-structured illumination microscopy. These phosphorescent dyes are part of a very exclusive group of transition-metal complexes that enable imaging beyond the diffraction limit. Triplet excited-state phosphorescence was also utilized to probe the O2 concentration at the mitochondria in vitro, using lifetime mapping techniques.

Item Type: Journal Article
Alternative Title:
Subjects: Q Science > QH Natural history
Divisions: Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH): Mitochondria, Electron microscopy
Journal or Publication Title: Inorganic Chemistry
Publisher: American Chemical Society
ISSN: 0020-1669
Official Date: 4 December 2017
Dates:
DateEvent
4 December 2017Published
22 November 2017Accepted
Volume: 56
Number: 24
Page Range: pp. 15259-15270
DOI: 10.1021/acs.inorgchem.7b02633
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
UNSPECIFIEDUniversity of Sheffieldhttp://dx.doi.org/10.13039/501100000858
654148 (Laserlab-Europe)Horizon 2020 Framework Programmehttp://dx.doi.org/10.13039/100010661

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