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Multimodal probes : superresolution and transmission electron microscopy imaging of mitochondria, and oxygen mapping of cells, using small-molecule Ir(III) luminescent complexes
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Shewring, Jonathan R., Cankut, Ahmet J., McKenzie, Luke K., Crowston, Bethany J., Botchway, Stanley W., Weinstein, Julia A., Edwards, Elizabeth and Ward, Michael D. (2017) Multimodal probes : superresolution and transmission electron microscopy imaging of mitochondria, and oxygen mapping of cells, using small-molecule Ir(III) luminescent complexes. Inorganic Chemistry, 56 (24). pp. 15259-15270. doi:10.1021/acs.inorgchem.7b02633
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WRAP-multi-modal-probes-super-resolution-TEM-imaging-mitochondria-Ward-2017.pdf - Accepted Version - Requires a PDF viewer. Download (11Mb) | Preview |
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Official URL: https://doi.org/10.1021/acs.inorgchem.7b02633
Abstract
We describe an Ir(III)-based small-molecule, multimodal probe for use in both light and electron microscopy. The direct correlation of data between light- and electron-microscopy-based imaging to investigate cellular processes at the ultrastructure level is a current challenge, requiring both dyes that must be brightly emissive for luminescence imaging and scatter electrons to give contrast for electron microscopy, at a single working concentration suitable for both methods. Here we describe the use of Ir(III) complexes as probes that provide excellent image contrast and quality for both luminescence and electron microscopy imaging, at the same working concentration. Significant contrast enhancement of cellular mitochondria was observed in transmission electron microscopy imaging, with and without the use of typical contrast agents. The specificity for cellular mitochondria was also confirmed with MitoTracker using confocal and 3D-structured illumination microscopy. These phosphorescent dyes are part of a very exclusive group of transition-metal complexes that enable imaging beyond the diffraction limit. Triplet excited-state phosphorescence was also utilized to probe the O2 concentration at the mitochondria in vitro, using lifetime mapping techniques.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QH Natural history | |||||||||
| Divisions: | Faculty of Science > Chemistry | |||||||||
| Library of Congress Subject Headings (LCSH): | Mitochondria, Electron microscopy | |||||||||
| Journal or Publication Title: | Inorganic Chemistry | |||||||||
| Publisher: | American Chemical Society | |||||||||
| ISSN: | 0020-1669 | |||||||||
| Official Date: | 4 December 2017 | |||||||||
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| Date of first compliant deposit: | 4 December 2017 | |||||||||
| Volume: | 56 | |||||||||
| Number: | 24 | |||||||||
| Page Range: | pp. 15259-15270 | |||||||||
| DOI: | 10.1021/acs.inorgchem.7b02633 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Restricted or Subscription Access | |||||||||
| RIOXX Funder/Project Grant: |
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