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Isolation and primary culture of various cell types from whole human endometrial biopsies
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Barros, Flavio, Brosens, Jan J. and Brighton, Paul (Paul J.) (2016) Isolation and primary culture of various cell types from whole human endometrial biopsies. Bio-protocol, 6 (22). e2028. doi:doi:10.21769/BioProtoc.2028
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Official URL: http://dx.doi.org/10.21769/BioProtoc.2028
Abstract
The isolation and primary culture of cells from human endometrial biopsies provides valuable experimental material for reproductive and gynaecological research. Whole endometrial biopsies are collected from consenting women and digested with collagenase and DNase I to dissociate cells from the extracellular matrix. Cell populations are then isolated through culturing, filtering and magnetic separation using cell-surface antigen markers. Here we provide a comprehensive protocol on how to isolate and culture individual cell types from whole endometrial tissues for use in in vitro experiments.
| Item Type: | Journal Article | ||||||
|---|---|---|---|---|---|---|---|
| Subjects: | R Medicine > RG Gynecology and obstetrics | ||||||
| Divisions: | Faculty of Medicine > Warwick Medical School | ||||||
| Library of Congress Subject Headings (LCSH): | Endometrium -- Biopsy -- Experiments | ||||||
| Journal or Publication Title: | Bio-protocol | ||||||
| Publisher: | Bio-protocol LLC | ||||||
| ISSN: | 2331-8325 | ||||||
| Official Date: | 20 November 2016 | ||||||
| Dates: |
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| Date of first compliant deposit: | 6 December 2017 | ||||||
| Volume: | 6 | ||||||
| Number: | 22 | ||||||
| Article Number: | e2028 | ||||||
| DOI: | doi:10.21769/BioProtoc.2028 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Restricted or Subscription Access |
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