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Isolation and primary culture of various cell types from whole human endometrial biopsies

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Barros, Flavio, Brosens, Jan J. and Brighton, Paul (Paul J.) (2016) Isolation and primary culture of various cell types from whole human endometrial biopsies. Bio-protocol, 6 (22). e2028. doi:doi:10.21769/BioProtoc.2028

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Official URL: http://dx.doi.org/10.21769/BioProtoc.2028

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Abstract

The isolation and primary culture of cells from human endometrial biopsies provides valuable experimental material for reproductive and gynaecological research. Whole endometrial biopsies are collected from consenting women and digested with collagenase and DNase I to dissociate cells from the extracellular matrix. Cell populations are then isolated through culturing, filtering and magnetic separation using cell-surface antigen markers. Here we provide a comprehensive protocol on how to isolate and culture individual cell types from whole endometrial tissues for use in in vitro experiments.

Item Type: Journal Article
Subjects: R Medicine > RG Gynecology and obstetrics
Divisions: Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Endometrium -- Biopsy -- Experiments
Journal or Publication Title: Bio-protocol
Publisher: Bio-protocol LLC
ISSN: 2331-8325
Official Date: 20 November 2016
Dates:
DateEvent
20 November 2016Published
5 October 2016Accepted
Date of first compliant deposit: 6 December 2017
Volume: 6
Number: 22
Article Number: e2028
DOI: doi:10.21769/BioProtoc.2028
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

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