Isolation of high molecular weight DNA from soil for cloning into BAC vectors
UNSPECIFIED. (2003) Isolation of high molecular weight DNA from soil for cloning into BAC vectors. FEMS MICROBIOLOGY LETTERS, 223 (1). pp. 15-20. ISSN 0378-1097Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/S0378-1097(03)00248-9
Isolation of high molecular weight DNA fragments from soil, in excess of 1 Mb, and of sufficient quality for cloning into an Escherichia coli-streptomycete artificial chromosome vector is described. The combination of indirect extraction of cells, using a nycodenz extraction technique, followed by lysis of biomass immobilised in agarose plugs, allowed fragments in excess of 1 Mb to be purified. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology|
|Journal or Publication Title:||FEMS MICROBIOLOGY LETTERS|
|Publisher:||ELSEVIER SCIENCE BV|
|Date:||6 June 2003|
|Number of Pages:||6|
|Page Range:||pp. 15-20|
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