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Isolation of high molecular weight DNA from soil for cloning into BAC vectors
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UNSPECIFIED (2003) Isolation of high molecular weight DNA from soil for cloning into BAC vectors. FEMS MICROBIOLOGY LETTERS, 223 (1). pp. 15-20. ISSN 0378-1097
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Official URL: http://dx.doi.org/10.1016/S0378-1097(03)00248-9
Abstract
Isolation of high molecular weight DNA fragments from soil, in excess of 1 Mb, and of sufficient quality for cloning into an Escherichia coli-streptomycete artificial chromosome vector is described. The combination of indirect extraction of cells, using a nycodenz extraction technique, followed by lysis of biomass immobilised in agarose plugs, allowed fragments in excess of 1 Mb to be purified. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QR Microbiology |
| Journal or Publication Title: | FEMS MICROBIOLOGY LETTERS |
| Publisher: | ELSEVIER SCIENCE BV |
| ISSN: | 0378-1097 |
| Date: | 6 June 2003 |
| Volume: | 223 |
| Number: | 1 |
| Number of Pages: | 6 |
| Page Range: | pp. 15-20 |
| Publication Status: | Published |
| URI: | http://wrap.warwick.ac.uk/id/eprint/9617 |
Data sourced from Thomson Reuters' Web of Knowledge
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