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Simera : modelling the PCR process to simulate realistic chimera formation
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Nichols, Ben and Quince, Christopher (2016) Simera : modelling the PCR process to simulate realistic chimera formation. Working Paper. bioRxiv: Cold Spring Harbour Laboratory. (Unpublished)
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Official URL: http://dx.doi.org/10.1101/072447
Abstract
Polymerase Chain Reaction (PCR) is the principal method of amplifying target DNA regions and, as such, is of great importance when performing microbial diversity studies. An unfortunate side effect of PCR is the formation of unwanted byproducts such as chimeras. The main goal of the work covered in this article is the development of an algorithm that simulates realistic chimeras for use in the evaluation of chimera detection software and for investigations into the accuracy of community structure analyses. Experimental data has helped to identify factors which may cause the formation of chimeras and has provided evidence of how influential these factors can be. This article makes use of some of this evidence in order to build a model with which to simulate the PCR process. This model helps to better explain the formation of chimeras and is therefore able to provide aid to future studies that intend to use PCR.
Item Type: | Working or Discussion Paper (Working Paper) | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Publisher: | Cold Spring Harbour Laboratory | ||||
Place of Publication: | bioRxiv | ||||
Book Title: | Simera: Modelling the PCR Process to Simulate Realistic Chimera Formation | ||||
Official Date: | 31 August 2016 | ||||
Dates: |
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DOI: | 10.1101/072447 | ||||
Institution: | University of Warwick | ||||
Status: | Not Peer Reviewed | ||||
Publication Status: | Unpublished | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
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