Pittera, Justine, Jouhet, Juliette, Breton, Solène, Garczarek, Laurence, Partensky, Frédéric, Maréchal, Éric, Nguyen, Ngoc A., Doré, Hugo, Ratin, Morgane, Pitt, Frances Diana, Scanlan, David J. and Six, Christophe (2017) Thermoacclimation and genome adaptation of the membrane lipidome in marine Synechococcus. Environmental Microbiology, 20 (2). pp. 612-631. doi:10.1111/1462-2920.13985 ISSN 1462-2912.

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Abstract

The marine cyanobacteria of the genus Synechococcus are important primary producers, displaying a wide latitudinal distribution that is underpinned by diversification into temperature ecotypes. The physiological basis underlying these ecotypes is poorly known. In many organisms, regulation of membrane fluidity is crucial for acclimating to variations in temperature. Here, we reveal the detailed composition of the membrane lipidome of the model strain Synechococcus sp. WH7803 and its response to temperature variation. Unlike freshwater strains, membranes are almost devoid of C18, mainly containing C14 and C16 chains with no more than two unsaturations. In response to cold, we observed a rarely observed process of acyl chain shortening that likely induces membrane thinning, along with specific desaturation activities. Both of these mechanisms likely regulate membrane fluidity, facilitating the maintenance of efficient photosynthetic activity. A comprehensive examination of 53 Synechococcus genomes revealed clade-specific gene sets regulating membrane lipids. In particular, the genes encoding desaturase enzymes, which is a key to the temperature stress response, appeared to be temperature ecotype-specific, with some of them originating from lateral transfers. Our study suggests that regulation of membrane fluidity has been among the important adaptation processes for the colonization of different thermal niches by marine Synechococcus.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Cyanobacteria -- genetitc aspects, Phytoplankton
Journal or Publication Title:	Environmental Microbiology
Publisher:	Blackwell
ISSN:	1462-2912
Official Date:	14 December 2017
Dates:	Date Event 14 December 2017 Published 10 November 2017 Available 2 November 2017 Accepted
Volume:	20
Number:	2
Page Range:	pp. 612-631
DOI:	10.1111/1462-2920.13985
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Date of first compliant deposit:	15 January 2018
Date of first compliant Open Access:	14 December 2018
Funder:	France. Government
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID ANR-13-ADAP-0010 Agence Nationale de la Recherche http://dx.doi.org/10.13039/501100001665 UNSPECIFIED Agence Nationale de la Recherche http://dx.doi.org/10.13039/501100001665 NE/I00985X/1 Natural Environment Research Council http://dx.doi.org/10.13039/501100000270 Investissement d’Avenir Oceanomics and Glyco@Alps Ministère de l'Enseignement Supérieur et de la Recherche Scientifique http://dx.doi.org/10.13039/501100002717 UNSPECIFIED European marine biological resource centre (EMBRC) UNSPECIFIED UNSPECIFIED France. Government UNSPECIFIED UNSPECIFIED Région Bretagne UNSPECIFIED

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