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Interaction between Staphylococcus aureus and Pseudomonas aeruginosa is beneficial for colonisation and pathogenicity in a mixed-biofilm
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Alves, Patrícia M., Al-Badi, Eide, Withycombe, Cathryn, Jones, Paul M, Purdy, Kevin J. and Maddocks, Sarah E. (2018) Interaction between Staphylococcus aureus and Pseudomonas aeruginosa is beneficial for colonisation and pathogenicity in a mixed-biofilm. Pathogens and disease, 76 (1). fty003. doi:10.1093/femspd/fty003 ISSN 2049-632X.
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Official URL: https://doi.org/10.1093/femspd/fty003
Abstract
Debate regarding the co-existence of Staphylococcus aureus and Pseudomonas aeruginosa in wounds remains contentious, with the dominant hypothesis describing a situation akin to niche partitioning, whereby both microorganisms are present but occupy distinct regions of the wound without interacting. In contrast, we hypothesised that these microorganisms do interact during early co-colonisation in a manner beneficial to both bacteria. We assessed competitive interaction between S. aureus and P. aeruginosa in biofilm cultured for 24-72 h and bacterial aggregates analogous to those observed in early ( < 24h) biofilm formation, and interaction with human keratinocytes. We observed that S. aureus predominated in biofilm and non-attached bacterial aggregates, acting as a pioneer for the attachment of P. aeruginosa. We report for the first time that S. aureus mediates a significant (P < 0.05) increase in the attachment of P. aeruginosa to human keratinocytes, and that P. aeruginosa promotes an invasive phenotype in S. aureus. We show that co-infected keratinocytes exhibit an intermediate inflammatory response concurrent with impaired wound closure that is in keeping with a sustained pro-inflammatory response which allows for persistent microbial colonisation. These studies demonstrate that, contrary to the dominant hypothesis, interactions between S. aureus and P. aeruginosa may be an important factor for both colonisation and pathogenicity in the chronic infected wound. [Abstract copyright: © FEMS 2018. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.]
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QR Microbiology | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| SWORD Depositor: | Library Publications Router | ||||||||
| Library of Congress Subject Headings (LCSH): | Staphylococcus aureus, Pseudomonas aeruginosa, Biofilms | ||||||||
| Journal or Publication Title: | Pathogens and disease | ||||||||
| Publisher: | Oxford University Press | ||||||||
| ISSN: | 2049-632X | ||||||||
| Official Date: | 1 February 2018 | ||||||||
| Dates: |
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| Volume: | 76 | ||||||||
| Number: | 1 | ||||||||
| Article Number: | fty003 | ||||||||
| DOI: | 10.1093/femspd/fty003 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Reuse Statement (publisher, data, author rights): | ** From PubMed via Jisc Publications Router. | ||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||
| Date of first compliant deposit: | 6 February 2018 | ||||||||
| Date of first compliant Open Access: | 12 January 2019 | ||||||||
| RIOXX Funder/Project Grant: |
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