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Nucleotide– and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics
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von Loeffelholz, Ottilie, Venables, Neil A., Drummond, Douglas R., Katsuki, Miho, Cross, R. A. and Moores, Carolyn A. (2017) Nucleotide– and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics. Nature Communications, 8 (1). 2110. doi:10.1038/s41467-017-02241-5 ISSN 2041-1723.
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Official URL: http://dx.doi.org/10.1038/s41467-017-02241-5
Abstract
Using cryo-electron microscopy, we characterize the architecture of microtubules assembled from Schizosaccharomyces pombe tubulin, in the presence and absence of their regulatory partner Mal3. Cryo-electron tomography reveals that microtubules assembled from S. pombe tubulin have predominantly B-lattice interprotofilament contacts, with protofilaments skewed around the microtubule axis. Copolymerization with Mal3 favors 13 protofilament microtubules with reduced protofilament skew, indicating that Mal3 adjusts interprotofilament interfaces. A 4.6-Å resolution structure of microtubule-bound Mal3 shows that Mal3 makes a distinctive footprint on the S. pombe microtubule lattice and that unlike mammalian microtubules, S. pombe microtubules do not show the longitudinal lattice compaction associated with EB protein binding and GTP hydrolysis. Our results firmly support a structural plasticity view of microtubule dynamics in which microtubule lattice conformation is sensitive to a variety of effectors and differently so for different tubulins.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QH Natural history | ||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Microtubules, Schizosaccharomyces pombe, Tubulins | ||||||||||||
| Journal or Publication Title: | Nature Communications | ||||||||||||
| Publisher: | Nature Publishing Group | ||||||||||||
| ISSN: | 2041-1723 | ||||||||||||
| Official Date: | 13 December 2017 | ||||||||||||
| Dates: |
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| Volume: | 8 | ||||||||||||
| Number: | 1 | ||||||||||||
| Article Number: | 2110 | ||||||||||||
| DOI: | 10.1038/s41467-017-02241-5 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
| Date of first compliant deposit: | 2 February 2018 | ||||||||||||
| Date of first compliant Open Access: | 2 February 2018 | ||||||||||||
| Funder: | University of Warwick. Systems Biology Doctoral Training Centre | ||||||||||||
| RIOXX Funder/Project Grant: |
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