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Evaluation of PCR as a diagnostic mass-screening tool to detect Leishmania (Viannia) spp. in domestic dogs (Canis familiaris)
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UNSPECIFIED. (2003) Evaluation of PCR as a diagnostic mass-screening tool to detect Leishmania (Viannia) spp. in domestic dogs (Canis familiaris). JOURNAL OF CLINICAL MICROBIOLOGY, 41 (4). pp. 1486-1493. ISSN 0095-1137
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Official URL: http://dx.doi.org/10.1128/JCM.41.4.1486-1493.2003
Abstract
Several studies have suggested that the PCR could be used in epidemiological mass-screening surveys to detect Leishmania (Piannia) spp. infection in human and animal hosts. Dogs from an area of Leishmania braziliensis and Leishmania peruviana endemicity were screened for American cutaneous leishmaniasis (ACL) infection by established PCR-based and enzyme-linked immunosorbent antibody test (ELISA) protocols. PCR detected Leishmania (Viannia) infection in a total of 90 of 1,066 (8.4%) dogs: 32 of 368 (8.7%), 65 of 769 (8.5%), and 7 of 42 (16.7%) dogs were PCR positive by testing of whole blood, bully coat, and bone marrow aspirates, respectively. ELISA detected infection in 221 of 1,059 (20.9%) tested dogs. The high prevalence of Leishmania (Viannia) detected by PCR and ELISA in both asymptomatic (7.5 and 19.2%, respectively) and symptomatic (32 and 62.5%, respectively) dogs is further circumstantial evidence for their suspected role as reservoir hosts of ACL. However, the low sensitivity of PCR (31%) compared to ELISA (81%) indicates that PCR cannot be used for mass screening of samples in ACL epidemiological studies. Unless more-sensitive PCR protocols were to be developed, its use should be restricted to the diagnosis of active (canine and human) cases and to the parasitological monitoring of patients after chemotherapy.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QR Microbiology |
| Journal or Publication Title: | JOURNAL OF CLINICAL MICROBIOLOGY |
| Publisher: | AMER SOC MICROBIOLOGY |
| ISSN: | 0095-1137 |
| Date: | April 2003 |
| Volume: | 41 |
| Number: | 4 |
| Number of Pages: | 8 |
| Page Range: | pp. 1486-1493 |
| Identification Number: | 10.1128/JCM.41.4.1486-1493.2003 |
| Publication Status: | Published |
| URI: | http://wrap.warwick.ac.uk/id/eprint/9832 |
Data sourced from Thomson Reuters' Web of Knowledge
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