Mohan, Thotegowdanapalya C. and Jones, Alexandra M. (2018) Determination of boron content using a simple and rapid miniaturized curcumin assay. Bio-protocol, 7 (2). doi:doi:10.21769/BioProtoc.2703

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Abstract

To determine boron quantity in soil, water and biological samples, several protocols are available. Colorimetric assays are the simplest and cheapest methods which can be used to determine boron concentration. However, published protocols do not give straightforward guidance for beginners to adopt these protocols for routine use in the laboratory. Based on a previously published available procedure, we present a detailed and modified version of a curcumin based colorimetric protocol to determine boron concentration extracted from any sample. Our modified protocol is able to determine up to 0.2 nmole of Boron in a sample volume of 300 µl.

Item Type:	Journal Article
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Soils -- Boron content -- Testing -- Methodology, Colorimetric analysis
Journal or Publication Title:	Bio-protocol
Publisher:	Bio-protocol LLC
ISSN:	2331-8325
Official Date:	20 January 2018
Dates:	Date Event 20 January 2018 Published 7 December 2017 Accepted
Date of first compliant deposit:	7 February 2018
Volume:	7
Number:	2
DOI:	doi:10.21769/BioProtoc.2703
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID BB/N017765/1 Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268

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