UNSPECIFIED. (2003) Haemophilus parainfluenzae infection of respiratory mucosa. RESPIRATORY MEDICINE, 97 (4). pp. 375-381. ISSN 0954-6111

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Abstract

The pathogenicity of Haemophilus parainfluenzae (Hpi) in the respiratory tract is unclear, in contrast to the accepted pathogenicity of its close relative non-typable H. influenzae. We have investigated the interaction of two Hpi isolates with the mucosa of adenoid and bronchial tissue organ cultures,The adherence of bacteria to the mucosa of organ cultures, the effect of broth culture filtrates on human nasal epithelium, and interleukin (IL)- 8 production by A549 cell cultures was investigated. Hpi 4846 adhered infrequently in clusters of pleomorphic cocco-bacilli to areas of epithelial damage, mucus and unciliated cells in adenoid organ culture experiments at 24 h, but not bronchial mucosa. Hpi 3698 was seen in only one adenoid and no bronchial organ cultures at 24 h. In separate experiments, Hpi 3698 was cleared more rapidly from the centre of the adenoid organ culture and was not cultured at 24 h. Although not adhering to the mucosa at 24 h, Hpi 3698, but not Hpi 4846, caused an increase in the amount of epithelial damage in both types of organ culture. Broth culture filtrates of both strains caused immediate slowing of ciliary beat frequency that progressed, and disrupted epithelial integrity Dialysed culture filtrates of both strains stimulated IL- 8 production by A549 cells, with the culture filtrate of Hpi 3698 being most potent. We conclude that two strains of Hpi varied in their adherence to adenoid tissue, and neither adhered to bronchial tissue. These results lead us to speculate that Hpi is only likely to be a pathogen in the lower respiratory tract when impaired airway defences delay bacterial clearance, (C) 2003 Elsevier Science Ltd. All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QM Human anatomy R Medicine > RC Internal medicine
Journal or Publication Title:	RESPIRATORY MEDICINE
Publisher:	W B SAUNDERS CO LTD
ISSN:	0954-6111
Date:	April 2003
Volume:	97
Number:	4
Number of Pages:	7
Page Range:	pp. 375-381
Identification Number:	10.1053/rmed.2002.1454
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/9867

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