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Serum albumin impedes the amyloid aggregation and hemolysis of human islet amyloid polypeptide and alpha synuclein
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Kakinen, Aleksandr, Javed, Ibrahim, Faridi, Ava, Davis, Thomas P. and Ke, Pu Chun (2018) Serum albumin impedes the amyloid aggregation and hemolysis of human islet amyloid polypeptide and alpha synuclein. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1860 (9). pp. 1803-1809. doi:10.1016/j.bbamem.2018.01.015 ISSN 0005-2736.
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WRAP-serum-albumin-impedes-amyloid-aggregation-hemolysis-human-Davis-2018.pdf - Accepted Version - Requires a PDF viewer. Download (1864Kb) | Preview |
Official URL: https://doi.org/10.1016/j.bbamem.2018.01.015
Abstract
Protein aggregation is a ubiquitous phenomenon underpinning the origins of a range of human diseases. The amyloid aggregation of human islet amyloid polypeptide (IAPP) and alpha synuclein (αS), specifically, is a hallmark of type 2 diabetes (T2D) and Parkinson's disease impacting millions of people worldwide. Although IAPP and αS are strongly associated with pancreatic β-cell islets and presynaptic terminals, they have also been found in blood circulation and the gut. While extensive biophysical and biochemical studies have been focused on IAPP and αS interacting with cell membranes or model lipid vesicles, the roles of plasma proteins on the amyloidosis and membrane association of these two major types of amyloid proteins have rarely been examined. Using a thioflavin T kinetic assay, transmission electron microscopy and a hemolysis assay here we show that human serum albumin, the most abundant protein in the plasma, impeded the fibrillization and mitigated membrane damage of both IAPP and αS. This study offers a new insight on the native inhibition of amyloidosis.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QP Physiology R Medicine > RC Internal medicine |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||
| Library of Congress Subject Headings (LCSH): | Amyloidosis -- Treatment, Serum albumin, Amyloid, Protein folding, Proteins -- Denaturation, Blood proteins | |||||||||
| Journal or Publication Title: | Biochimica et Biophysica Acta (BBA) - Biomembranes | |||||||||
| Publisher: | Elsevier BV | |||||||||
| ISSN: | 0005-2736 | |||||||||
| Official Date: | September 2018 | |||||||||
| Dates: |
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| Volume: | 1860 | |||||||||
| Number: | 9 | |||||||||
| Page Range: | pp. 1803-1809 | |||||||||
| DOI: | 10.1016/j.bbamem.2018.01.015 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Restricted or Subscription Access | |||||||||
| Date of first compliant deposit: | 15 February 2018 | |||||||||
| Date of first compliant Open Access: | 31 January 2019 | |||||||||
| RIOXX Funder/Project Grant: |
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