The Library
Phylogenic characteristics of a unique antagonistic micromonospora Sp. Rc5 to S. aureus isolated from Sinai Desert of Egypt
Tools
Tools
Tools
Amin, Dina, Abolmaaty, Assem , Tolba, Sahar , Abdallah, Nagwa and Wellington, E. M. H. (2018) Phylogenic characteristics of a unique antagonistic micromonospora Sp. Rc5 to S. aureus isolated from Sinai Desert of Egypt. Annual Research & Review in Biology, 22 (2). pp. 1-15. doi:10.9734/ARRB/2018/38318 ISSN 2347-565X.
|
PDF
WRAP-phylogenic-unique-isolated-Egypt-Wellington-2018.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (883Kb) | Preview |
Official URL: http://dx.doi.org/10.9734/ARRB/2018/38318
Abstract
A distinct strain named Micromonospora sp. Rc5 was isolated from Sinai desert of Egypt and recorded high antagonistic activities against some food and bloodborne pathogens. Morphological and chemotaxonomy characterization confirmed that this isolate belongs to genus Micromonospora. Sequencing of partial 16S rDNA and BLASTN showed that isolate Rc5 is identical to Micromonospora haikouensis (99%) but with low bootstrap value in NJ phylogenetic tree. Comprehensive optimization of several growth factors was performed including initial pH, incubation periods, and different sources of carbon and nitrogen. The highest yield of antimicrobial agent production was obtained after 8 days of incubation at 30°C, pH 6.0, 3 x 105CFU/ml in soya bean meal broth media with agitation of 150 rpm. A dramatic proportional decrease occurred with 0.3, 0.6, 0.9 µg active fraction /ml against Staphylococcus aureus culture and reached to complete inhibition at a minimum inhibitory concentration of (1.5 µg /ml). The physicochemical characterization of the purified fraction was identified as phthalate derivative. Our results indicated that Rc5 generated potential antimicrobial compounds against foodborne pathogens and may combat resistant strains of Staphylococcus aureus.
| Item Type: | Journal Article | ||||||
|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QR Microbiology | ||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
| Library of Congress Subject Headings (LCSH): | Staphylococcus aureus, Micromonospora, Phthalate esters | ||||||
| Journal or Publication Title: | Annual Research & Review in Biology | ||||||
| Publisher: | Science Domain International | ||||||
| ISSN: | 2347-565X | ||||||
| Official Date: | 9 January 2018 | ||||||
| Dates: |
|
||||||
| Volume: | 22 | ||||||
| Number: | 2 | ||||||
| Page Range: | pp. 1-15 | ||||||
| DOI: | 10.9734/ARRB/2018/38318 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||
| Date of first compliant deposit: | 21 February 2018 | ||||||
| Date of first compliant Open Access: | 21 February 2018 | ||||||
| RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
