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Regulation of corticotropin-releasing hormone type 2 receptors by multiple promoters and alternative splicing: Identification of multiple splice variants
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UNSPECIFIED. (2003) Regulation of corticotropin-releasing hormone type 2 receptors by multiple promoters and alternative splicing: Identification of multiple splice variants. MOLECULAR ENDOCRINOLOGY, 17 (3). pp. 395-410. ISSN 0888-8809
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Official URL: http://dx.doi.org/10.1210/me.2002.0302
Abstract
We demonstrate that multiple promoters and alternate splicing regulate expression-of the human CRH receptor type 2 (CRHR2) gene. We show that flanking regions to the first exons drive promoter activity in both endogenously and nonendogenously expressing cell lines. Putative promoter elements have been identified that are conserved between species, including the comparison of CRHR2(gamma) in nonhuman primates that was previously known only in humans, which may be responsible for subtype tissue specific regulation. We have identified novel transcripts produced by alternate splicing of the first exon of CRHR2(beta) (beta1a) with various combinations of the 5' exons including a novel exon (beta1c) spliced to the common exons. The 5' structure of the gene permits many other combinations of alternate splicing that may arise as part Of a regulatory mechanism controlling functional receptor expression. The 5'-untranslated region of the first exons has been extended; and 3' acceptor sites identified within the 5' untranslated region of CRHR2(gamma) and CRHR2(alpha) are used during alternate splicing of CRHR2(beta) upstream exons. This has important implications because various reports on the expression of CRHR2(gamma) and CRHR2(alpha). have been unable to discriminate between the functional receptor and CRHR2(beta) alternate splice variants. Only the described sequences upstream of the 3' splice site are unique to CRHR2(gamma) and CRHR2(alpha)..
| Item Type: | Journal Article |
|---|---|
| Subjects: | R Medicine > RC Internal medicine |
| Journal or Publication Title: | MOLECULAR ENDOCRINOLOGY |
| Publisher: | ENDOCRINE SOC |
| ISSN: | 0888-8809 |
| Date: | March 2003 |
| Volume: | 17 |
| Number: | 3 |
| Number of Pages: | 16 |
| Page Range: | pp. 395-410 |
| Identification Number: | 10.1210/me.2002.0302 |
| Publication Status: | Published |
| URI: | http://wrap.warwick.ac.uk/id/eprint/9943 |
Data sourced from Thomson Reuters' Web of Knowledge
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