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A comparison of ergosterol and PLFA methods for monitoring the growth of ligninolytic fungi during wheat straw solid state fermentation
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Nurika, Irnia, Eastwood, Daniel C. and Barker, Guy C. (2018) A comparison of ergosterol and PLFA methods for monitoring the growth of ligninolytic fungi during wheat straw solid state fermentation. Journal of Microbiological Methods, 148 . pp. 49-54. doi:10.1016/j.mimet.2018.03.006
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Official URL: https://doi.org/10.1016/j.mimet.2018.03.006
Abstract
Ergosterol, total phospholipid fatty acid (PLFA) and linoleic acid (18:2n-6) have all been used to determine fungal growth. This paper compares these methods to assess the growth of four different saprotrophic fungal species during solid state cultivation using a wheat straw substrate that have not been compared or measured previously. Ergosterol production appeared to track the mycelia growth well but its production differed considerably between fungi. This means that a specific conversion factor needs to be determined and applied for any given fungus. In comparison, measurements of total PLFA and linoleic acid only showed promise for determining the growth of Postia placenta due to the positive correlation with ergosterol measurements. In contrast, the other fungi tested (Phanerochaete chrysosporium, Serpula lacrymans and Schizophyllum commune) showed either no correlation or in some cases a negative correlation using this assay. The novel findings highlight the variation in fungal fatty acid between species, culture conditions and durations of incubation; suggesting that measurement of linoleic acid is only usable in specific cases. These findings provide important consideration for the study of fungi growing in solid substrates and suggest that the use of PLFA might bias diversity indices.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | S Agriculture > SB Plant culture T Technology > TP Chemical technology |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Biomass, Wheat straw, Ergosterol, Phospholipids, Fungi -- Growth, linoleic acid | ||||||||||||
| Journal or Publication Title: | Journal of Microbiological Methods | ||||||||||||
| Publisher: | Elsevier | ||||||||||||
| ISSN: | 0167-7012 | ||||||||||||
| Official Date: | May 2018 | ||||||||||||
| Dates: |
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| Date of first compliant deposit: | 15 March 2018 | ||||||||||||
| Volume: | 148 | ||||||||||||
| Page Range: | pp. 49-54 | ||||||||||||
| DOI: | 10.1016/j.mimet.2018.03.006 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
| RIOXX Funder/Project Grant: |
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