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DmsD is required for the biogenesis of DMSO reductase in Escherichia coli but not for the interaction of the DmsA signal peptide with the Tat apparatus
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UNSPECIFIED (2003) DmsD is required for the biogenesis of DMSO reductase in Escherichia coli but not for the interaction of the DmsA signal peptide with the Tat apparatus. FEBS LETTERS, 534 (1-3). pp. 156-160. doi:10.1016/S0014-5793(02)03839-5 ISSN 0014-5793.
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Official URL: http://dx.doi.org/10.1016/S0014-5793(02)03839-5
Abstract
The DmsD protein is essential for the biogenesis of DMSO reductase in Escherichia coli, and binds the signal peptide of the DmsA subunit, a Tat substrate. This suggests a role as a guidance factor to target pre-DmsA to the translocase. Here, we have analysed the export of fusion proteins in which the DmsA and TorA signal peptides are fused to green fluorescent protein. Both chimeras are efficiently exported to the periplasm in wild-type E. coli cells and we show that their export efficiencies are essentially identical in a mutant lacking DmsD. An authentic Tat substrate, TMAO reductase, is also efficiently exported in the dmsD mutant. The data indicate that DmsD carries out a critical role in DMSO reductase biogenesis/assembly but is not required for the functioning of the DmsA signal peptide. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
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Journal or Publication Title: | FEBS LETTERS | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0014-5793 | ||||
Official Date: | 16 January 2003 | ||||
Dates: |
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Volume: | 534 | ||||
Number: | 1-3 | ||||
Number of Pages: | 5 | ||||
Page Range: | pp. 156-160 | ||||
DOI: | 10.1016/S0014-5793(02)03839-5 | ||||
Publication Status: | Published |
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