The Library
Selectivity studies of the benzene cis-dihydrodiol dehydrogenase enzyme from Pseudomonas putida ML2 with Vicinal diol substrates
Tools
UNSPECIFIED (2002) Selectivity studies of the benzene cis-dihydrodiol dehydrogenase enzyme from Pseudomonas putida ML2 with Vicinal diol substrates. BIOCATALYSIS AND BIOTRANSFORMATION, 20 (4). pp. 257-264. doi:10.1080/10242420290029454 ISSN 1024-2422.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1080/10242420290029454
Abstract
The enantiopure (1S, 2S)-cis-dihydrodiol metabolites 2B-5B have been obtained in low yield from the corresponding monosubstituted halobenzene substrates 2A-5A, using a wild-type strain of Pseudomonas putida (ML2) containing benzene dioxygenase (BDO). Benzene cis-dihydrodiol dehydrogenase (BCD) from P. putida ML2 and naphthalene cis-dihydrodiol dehydrogenase (NCD) from P. putida 8859 were purified and used in a comparative study of the stereoselective biotransformation of cis-dihydrodiol enantiomers 2B-5B. The BCD and NCD enzymes were found to accept cis-dihydrodiol enantiomers of monosubstituted benzene cis-dihydrodiol substrates 2B-5B of opposite absolute configuration. The acyclic alkene 1,2-diols 10-17 were also found to be acceptable substrates for BCD.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry T Technology > TP Chemical technology |
||||
Journal or Publication Title: | BIOCATALYSIS AND BIOTRANSFORMATION | ||||
Publisher: | TAYLOR & FRANCIS LTD | ||||
ISSN: | 1024-2422 | ||||
Official Date: | August 2002 | ||||
Dates: |
|
||||
Volume: | 20 | ||||
Number: | 4 | ||||
Number of Pages: | 8 | ||||
Page Range: | pp. 257-264 | ||||
DOI: | 10.1080/10242420290029454 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |