The Library
5-methylthiopentose: a new substituent on lipoarabinomannan in Mycobacterium tuberculosis
Tools
UNSPECIFIED (2002) 5-methylthiopentose: a new substituent on lipoarabinomannan in Mycobacterium tuberculosis. Journal of Molecular Biology, 316 (1). pp. 89-100. doi:10.1006/jmbi.2001.5317 ISSN 0022-2836.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1006/jmbi.2001.5317
Abstract
We have identified and characterised in several strains of Mycobacterium tuberculosis a new 5-methylthiopentose substituent on lipoarabinomannan (LAM). The 5-methylthiopentose was initially observed in heteronuclear H-1-C-13-NMR spectra of intact, C-13-enriched LAM. Oligosaccharides carrying this substituent were released from C-13-enriched LAM and from unlabelled LAM using an endo-arabinanase from Cellulomonas gellida. The presence of the methylthio group in these oligosaccharides was established using NMR, high-resolution Fourier-transform ion cyclotron resonance mass spectrometry and tandem mass spectrometry using a Q-TOF mass spectrometer. The 5-methylthiopentose is linked to a terminal mannose in the cap structures of these oligosaccharides as evidenced by tandem mass spectrometry and by NMR. We suggest interference with the signal transduction mechanisms of infected macrophages as a possible function for this newly discovered LAM substituent. (C) 2002 Elsevier Science Ltd.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | Journal of Molecular Biology | ||||
Publisher: | ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD | ||||
ISSN: | 0022-2836 | ||||
Official Date: | 8 February 2002 | ||||
Dates: |
|
||||
Volume: | 316 | ||||
Number: | 1 | ||||
Number of Pages: | 12 | ||||
Page Range: | pp. 89-100 | ||||
DOI: | 10.1006/jmbi.2001.5317 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |