The Library
The internal propeptide of the ricin precursor carries a sequence-specific determinant for vacuolar sorting
Tools
UNSPECIFIED (2001) The internal propeptide of the ricin precursor carries a sequence-specific determinant for vacuolar sorting. PLANT PHYSIOLOGY, 126 (1). pp. 167-175. ISSN 0032-0889.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Abstract
Ricin is a heterodimeric toxin that accumulates in the storage vacuoles of castor bean (Ricinus communis) endosperm. Proricin is synthesized as a single polypeptide precursor comprising the catalytic A chain and the Gal-binding B chain joined by a 12-amino acid linker propeptide. Upon arrival in the vacuole, the linker is removed. Here, we replicate these events in transfected tobacco (Nicotiana tabacum) leaf protoplasts. We show that the internal linker propeptide is responsible for vacuolar sorting and is sufficient to redirect the ricin heterodimer to the vacuole when fused to the A or the B chain. This internal peptide can also target two different secretory protein reporters to the vacuole. Moreover, mutation of the isoleucine residue within an NPIR-like motif of the propeptide affects vacuolar sorting in proricin and in the reconstituted A-B heterodimer. This is the first reported example of a sequence-specific vacuolar sorting signal located within an internal propeptide.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | S Agriculture > SB Plant culture | ||||
Journal or Publication Title: | PLANT PHYSIOLOGY | ||||
Publisher: | AMER SOC PLANT PHYSIOLOGISTS | ||||
ISSN: | 0032-0889 | ||||
Official Date: | May 2001 | ||||
Dates: |
|
||||
Volume: | 126 | ||||
Number: | 1 | ||||
Number of Pages: | 9 | ||||
Page Range: | pp. 167-175 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |