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High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector
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UNSPECIFIED (2001) High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector. Biotechnology Letters, 23 (8). pp. 643-646. ISSN 0141-5492.
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Abstract
Six regions of the VP4 protein of bovine rotavirus strain UKtc were expressed using hepatitis B core antigen (HBcAg) as a carrier. Following induction by IPTG, the six fusion proteins, AHBcAg through FHBcAg, were expressed in Escherichia coli to a level of 20-37% of total cellular protein. Soluble fusion proteins in a particulate form were partially purified with yields ranging from 0.5-6.4 mg l(-1) of culture.
Item Type: | Journal Article | ||||
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Subjects: | T Technology > TP Chemical technology | ||||
Journal or Publication Title: | Biotechnology Letters | ||||
Publisher: | KLUWER ACADEMIC PUBL | ||||
ISSN: | 0141-5492 | ||||
Official Date: | April 2001 | ||||
Dates: |
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Volume: | 23 | ||||
Number: | 8 | ||||
Number of Pages: | 4 | ||||
Page Range: | pp. 643-646 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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