The Library
Heterocomplex formation between metastasis-related protein S100A4 (Mts1) and S100A1 as revealed by the yeast two-hybrid system
Tools
UNSPECIFIED (2000) Heterocomplex formation between metastasis-related protein S100A4 (Mts1) and S100A1 as revealed by the yeast two-hybrid system. FEBS LETTERS, 475 (3). pp. 187-191. ISSN 0014-5793.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Abstract
S100A4 (Mts1) is a Ca2+-binding protein of the S100 family. This protein plays an important role in promoting tumor metastasis, In order to identify S100A4 interacting proteins, we have applied the yeast two-hybrid system as an in vivo approach. By screening a mouse mammary adenocarcinoma library, we have demonstrated that S100A4 forms a heterocomplex with S100A1, another member of the S100 family, The non-covalent heterodimerization was confirmed by fluorescence spectroscopy and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry, Mutational analysis revealed that replacement of Cys(76) and/or Cys(81) of S100A4 by Ser abolishes the S100A4/S100A1 heterodimerization, but does not affect the S100A4 homodimerization in vivo. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
||||
Journal or Publication Title: | FEBS LETTERS | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0014-5793 | ||||
Official Date: | 23 June 2000 | ||||
Dates: |
|
||||
Volume: | 475 | ||||
Number: | 3 | ||||
Number of Pages: | 5 | ||||
Page Range: | pp. 187-191 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |