The Library
Unfolding and refolding of cytochrome c driven by the interaction with lipid micelles
Tools
UNSPECIFIED (2000) Unfolding and refolding of cytochrome c driven by the interaction with lipid micelles. PROTEIN SCIENCE, 9 (6). pp. 1194-1202. ISSN 0961-8368.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Abstract
Binding of native cyt c to L-PG micelles leads to a partially unfolded conformation of cyt c. This micelle-bound state has no stable tertiary structure. but remains as cu-helical as native cvt c in solution. In contrast, binding of the acid-unfolded cvt c to L-PG micelles induces folding of the polypeptide. resulting in a similar helical state to that originated from the binding of native cyt c to L-PG micelles. Far-ultraviolet (UV) circular dichroism (CD) spectra showed that this common micelle-associated helical state (H-L) has a native-like alpha-helix content, but is highly expanded without a tightly packed hydrophobic core, as revealed by tryptophan fluorescence, near-UV, and Sent CD spectroscopy. The kinetics of the interaction of native and acid-unfolded cyt c was investigated by stopped-flow tryptophan fluorescence. Formation of HL from the native state requires the disruption of the tightly packed hydrophobic core in the native protein. This micelle-induced unfolding of cyt c occurs at a rate similar to 0.1 s(-1), which is remarkably faster in the lipid environment compared with the expected rate of unfolding in solution. Refolding of acid-unfolded cyt c with L-PG micelles involves an early highly helical collapsed state formed during the burst phase (<3 ms), and the observed main kinetic event reports on the opening of this early compact intermediate prior to insertion into the lipid micelle.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | PROTEIN SCIENCE | ||||
Publisher: | CAMBRIDGE UNIV PRESS | ||||
ISSN: | 0961-8368 | ||||
Official Date: | June 2000 | ||||
Dates: |
|
||||
Volume: | 9 | ||||
Number: | 6 | ||||
Number of Pages: | 9 | ||||
Page Range: | pp. 1194-1202 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |